FEBS letters, 2016-12, Vol.590 (24), p.4573-4585
2016
Volltextzugriff (PDF)
Details
- Autor(en) / Beteiligte
- Titel
- Characterizing polyubiquitinated forms of the neurodegenerative ubiquitin mutant UBB+1
- Ist Teil von
- FEBS letters, 2016-12, Vol.590 (24), p.4573-4585
- Ort / Verlag
- England
- Erscheinungsjahr
- 2016
- Quelle
- Wiley Online Library All Journals
- Beschreibungen/Notizen
- The ubiquitin mutant UBB+1 has been identified as a hallmark of neurodegenerative diseases. In this study, we characterize polyubiquitinated forms of UBB+1 in vitro and from patient samples. The ability of UBB+1 to be readily ubiquitinated by several E2 enzymes provided a mechanism for the controlled synthesis and purification of defined conjugates. This allowed us to utilize polyUb–UBB+1 conjugates for biochemical assays, as well as solution NMR. Coupled with our immunoassay for detection of ubiquitinated forms of UBB+1 in patient blood samples, we gain a clearer picture of the molecular mechanisms underlying neurodegenerative diseases.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0014-5793eISSN: 1873-3468DOI: 10.1002/1873-3468.12484Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5182158
- Format
- –
- Schlagworte
- Aged, Alzheimer Disease - genetics, Alzheimer Disease - metabolism, Alzheimer Disease - pathology, biomolecular NMR, Case-Control Studies, Cysteine Endopeptidases - chemistry, Cysteine Endopeptidases - genetics, Cysteine Endopeptidases - metabolism, deubiquitinase, Endopeptidases - chemistry, Endopeptidases - genetics, Endopeptidases - metabolism, Endosomal Sorting Complexes Required for Transport - chemistry, Endosomal Sorting Complexes Required for Transport - genetics, Endosomal Sorting Complexes Required for Transport - metabolism, Female, Fluoresceins - chemistry, Fluorescent Dyes - chemistry, Gene Expression Regulation, Humans, Male, Middle Aged, Models, Molecular, neurodegeneration, Neurons - metabolism, Neurons - pathology, Nuclear Magnetic Resonance, Biomolecular, polyubiquitin, Polyubiquitin - chemistry, Polyubiquitin - genetics, Polyubiquitin - metabolism, Proteasome Endopeptidase Complex - metabolism, Protein Isoforms - chemistry, Protein Isoforms - genetics, Protein Isoforms - metabolism, Protein Structure, Secondary, Staining and Labeling, UBB +1, Ubiquitin - chemistry, Ubiquitin - genetics, Ubiquitin - metabolism, Ubiquitin Thiolesterase - chemistry, Ubiquitin Thiolesterase - genetics, Ubiquitin Thiolesterase - metabolism, Ubiquitin-Specific Proteases - chemistry, Ubiquitin-Specific Proteases - genetics, Ubiquitin-Specific Proteases - metabolism, Ubiquitination