Details

Autor(en) / Beteiligte

• Wei, Yuan
• Qiu, Wei
• Zhou, Xue-Dong
• Zheng, Xin
• Zhang, Ke-Ke
• Wang, Shi-Da
• Li, Yu-Qing
• Cheng, Lei
• Li, Ji-Yao
• Xu, Xin
• Li, Ming-Yun

Titel

Alanine racemase is essential for the growth and interspecies competitiveness of Streptococcus mutans

Ist Teil von

• International journal of oral science, 2016-12, Vol.8 (4), p.231-238

Ort / Verlag

India: Springer Nature B.V

Erscheinungsjahr

2016

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• D-alanine （D-Ala） is an essential amino acid that has a key role in bacterial unique enzyme that interconverts L-alanine and D-alanine in most bacteria, antimicrobial drug development. Streptococcus mutans is a major causative cell wall synthesis. Alanine racemase （Air） is a making this enzyme a potential target for factor of dental caries. The factors involved in the survival, virulence and interspecies interactions of S. mutans could be exploited as potential targets for caries control. The current study aimed to investigate the physiological role of Air in S. mutans. We constructed air mutant strain of S. mutans and evaluated its phenotypic traits and interspecies competitiveness compared with the wild-type strain. We found that air deletion was lethal to S. mutans. A minimal supplement of D-Ala （150 pg.mL- 1） was required for the optimal growth of the air mutant. The depletion of D-alanine in the growth medium resulted in cell wall perforation and cell lysis in the air mutant strain. We also determined the compromised competitiveness of the air mutant strain relative to the wild-type S. mutans against other oral streptococci （S. sanguinis or S. gordonil~, demonstrated using either conditioned medium assays or dual-species fluorescent in situ hybridization analysis. Given the importance and necessity of air to the growth and competitiveness of S. mutans, Air may represent a promising target to modulate the cariogenicity of oral biofilms and to benefit the management of dental caries.