The EMBO journal, 2004-08, Vol.23 (16), p.3336-3345
2004
Details
- Autor(en) / Beteiligte
- Titel
- Regulation of human SRY subcellular distribution by its acetylation/deacetylation
- Ist Teil von
- The EMBO journal, 2004-08, Vol.23 (16), p.3336-3345
- Ort / Verlag
- Chichester, UK: John Wiley & Sons, Ltd
- Erscheinungsjahr
- 2004
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- SRY, a Y chromosome‐encoded DNA‐binding protein, is required for testis organogenesis in mammals. Expression of the SRY gene in the genital ridge is followed by diverse early cell events leading to Sertoli cell determination/differentiation and subsequent sex cord formation. Little is known about SRY regulation and its mode of action during testis development, and direct gene targets for SRY are still lacking. In this study, we demonstrate that interaction of the human SRY with histone acetyltransferase p300 induces the acetylation of SRY both in vitro and in vivo at a single conserved lysine residue. We show that acetylation participates in the nuclear localisation of SRY by increasing SRY interaction with importin β, while specific deacetylation by HDAC3 induces a cytoplasmic delocalisation of SRY. Finally, by analysing p300 and HDAC3 expression profiles during both human or mouse gonadal development, we suggest that acetylation and deacetylation of SRY may be important mechanisms for regulating SRY activity during mammalian sex determination.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0261-4189eISSN: 1460-2075DOI: 10.1038/sj.emboj.7600352Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_514523
- Format
- –
- Schlagworte
- Acetylation, Acetyltransferases - metabolism, Active Transport, Cell Nucleus, Animals, Cell Cycle Proteins - metabolism, Cell Line, Cell Nucleus - metabolism, DNA - metabolism, DNA-Binding Proteins - genetics, DNA-Binding Proteins - metabolism, Gene Expression Regulation, Gonads - embryology, Gonads - metabolism, Histone Acetyltransferases, Histone Deacetylases - metabolism, Humans, Lysine - genetics, Lysine - metabolism, Male, mammals, Mice, nuclear localisation, Nuclear Proteins - genetics, Nuclear Proteins - metabolism, p300-CBP Transcription Factors, Protein Binding, sex determination, Sex-Determining Region Y Protein, SRY, Transcription Factors - genetics, Transcription Factors - metabolism