Science (American Association for the Advancement of Science), 2004-03, Vol.303 (5666), p.2022-2025
2004
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Details
- Autor(en) / Beteiligte
- Titel
- A MicroRNA as a Translational Repressor of APETALA2 in Arabidopsis Flower Development
- Ist Teil von
- Science (American Association for the Advancement of Science), 2004-03, Vol.303 (5666), p.2022-2025
- Ort / Verlag
- Washington, DC: American Association for the Advancement of Science
- Erscheinungsjahr
- 2004
- Quelle
- American Association for the Advancement of Science
- Beschreibungen/Notizen
- Plant microRNAs (miRNAs) show a high degree of sequence complementarity to, and are believed to guide the cleavage of, their target messenger RNAs. Here, I show that miRNA172, which can base-pair with the messenger RNA of a floral homeotic gene, APETALA2, regulates APETALA2 expression primarily through translational inhibition. Elevated miRNA172 accumulation results in floral organ identity defects similar to those in loss-of-function apetala2 mutants. Elevated levels of mutant APETALA2 RNA with disrupted miRNA172 base pairing, but not wild-type APETALA2 RNA, result in elevated levels of APETALA2 protein and severe floral patterning defects. Therefore, miRNA172 likely acts in cell-fate specification as a translational repressor of APETALA2 in Arabidopsis flower development.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0036-8075eISSN: 1095-9203DOI: 10.1126/science.1088060Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5127708
- Format
- –
- Schlagworte
- Antisense Elements (Genetics), APETALA2 gene, Arabidopsis, Arabidopsis - genetics, Arabidopsis - growth & development, Arabidopsis - metabolism, Arabidopsis Proteins - genetics, Arabidopsis Proteins - metabolism, Arabidopsis Proteins - physiology, Base Pairing, Binding Sites, Biological and medical sciences, Biology and morphogenesis of the reproductive apparatus. Photoperiodism, vernalisation, Coding, Company growth, Flowering, Flowers, Flowers & plants, Flowers - anatomy & histology, Flowers - growth & development, Fundamental and applied biological sciences. Psychology, Gene expression regulation, Gene Expression Regulation, Plant, Genes, Genes, Homeobox, Genes, Plant, Genetic aspects, Growth, Homeodomain Proteins - genetics, Homeodomain Proteins - metabolism, In Situ Hybridization, Leaves, Messenger RNA, MicroRNA, MicroRNAs - chemistry, MicroRNAs - genetics, MicroRNAs - metabolism, miRNA, Mutation, Nuclear Proteins - genetics, Nuclear Proteins - metabolism, Phenotype, Phenotypes, Physiological aspects, Plant physiology and development, Plant Proteins, Plants, Plants, Genetically Modified, Protein Biosynthesis, Ribonucleic acid, RNA, RNA, Messenger - chemistry, RNA, Messenger - metabolism, RNA, Plant - chemistry, RNA, Plant - genetics, Transgenic plants, Vegetative and sexual reproduction, floral biology, fructification