Molecular medicine reports, 2016-11, Vol.14 (5), p.4055-4062
2016
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- Autor(en) / Beteiligte
- Titel
- Involvement of peroxisome proliferator activated receptor-γ in the anti-inflammatory effects of atorvastatin in oxygen-glucose deprivation/reperfusion-stimulated RAW264.7 murine macrophages
- Ist Teil von
- Molecular medicine reports, 2016-11, Vol.14 (5), p.4055-4062
- Ort / Verlag
- Greece: D.A. Spandidos
- Erscheinungsjahr
- 2016
- Quelle
- Elektronische Zeitschriftenbibliothek - Freely accessible e-journals
- Beschreibungen/Notizen
- Ischemia-reperfusion (I/R) injury is important in the pathogenesis and/or progression of various diseases, including stroke, cardiovascular disease and acute renal injury. Increasing evidence indicates that atorvastatin exerts protective effects in I/R injury-associated diseases; however, the underlying mechanisms remain to be fully elucidated. In the present study, oxygen-glucose deprivation (OGD)/reperfusion-stimulated. RAW264.7 murine macrophages served as a model of I/R injury. The knockdown of peroxisome proliferator activated receptor-γ (PPARγ) expression in these cells increased OGD/reperfusion-induced expression of inducible nitric oxide synthase (iNOS), tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-γ), and enhanced OGD/reperfusion-induced downregulation of the expression of cluster of differentiation (CD) 206, at the mRNA and protein levels. Conversely, overexpression of PPARγ significantly attenuated OGD/reperfusion-induced alterations in the expression of iNOS, TNF-α, IFN-γ and CD206 at the mRNA and protein levels. Notably, atorvastatin inhibited OGD/reperfusion-induced iNOS expression and reversed OGD/reperfusion-induced downregulation of the expression of CD206 and PPARγ at the mRNA and protein levels. The results of the present study indicate that atorvastatin exhibits significant anti-inflammatory effects in OGD/reperfusion-stimulated RAW264.7 cells, possibly via PPARγ regulation. The findings of the present study may reveal a novel mechanism underlying the protective effects of atorvastatin in I/R injury-associated diseases.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1791-2997eISSN: 1791-3004DOI: 10.3892/mmr.2016.5742Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5101877
- Format
- –
- Schlagworte
- Animals, Atorvastatin, Atorvastatin Calcium - administration & dosage, Cardiovascular disease, Cell culture, Cytokines, Development and progression, Disease Models, Animal, Gene Expression Regulation - drug effects, Gene Knockdown Techniques, Genetic aspects, Genotype & phenotype, Glucose, Glucose - metabolism, Health aspects, Humans, Immunoglobulins, Inflammation, Inflammation - drug therapy, Inflammation - genetics, Inflammation - pathology, Interferon-gamma - biosynthesis, Ischemia, Lectins, C-Type - biosynthesis, Macrophages, Mannose-Binding Lectins - biosynthesis, Mice, mRNA, Nitric oxide, Nitric Oxide Synthase Type II - biosynthesis, Nitric-oxide synthase, Oxygen, Oxygen - metabolism, oxygen-glucose deprivation/reperfusion, peroxisome proliferator activated receptor-γ, Plasmids, PPAR gamma - antagonists & inhibitors, PPAR gamma - genetics, RAW 264.7 Cells, Receptors, Cell Surface - biosynthesis, Reperfusion, Reperfusion injury, Reperfusion Injury - drug therapy, Reperfusion Injury - genetics, Reperfusion Injury - pathology, Rodents, Stroke, Studies, Transcription factors, Tumor Necrosis Factor-alpha - biosynthesis, Tumor necrosis factor-TNF, Tumor necrosis factor-α, γ-Interferon