Details

Autor(en) / Beteiligte

• Cimolai, N
• Trombley, C
• Davidson, A G
• Wong, L T

Titel

Selective media for isolation of Burkholderia (Pseudomonas) cepacia from the respiratory secretions of patients with cystic fibrosis

Ist Teil von

• Journal of clinical pathology, 1995-05, Vol.48 (5), p.488-490

Ort / Verlag

London: BMJ Publishing Group Ltd and Association of Clinical Pathologists

Erscheinungsjahr

1995

Quelle

MEDLINE

Beschreibungen/Notizen

• One hundred and six specimens from 90 patients with cystic fibrosis were evaluated for the presence of Burkholderia cepacia using a current routine diagnostic protocol as well as a research protocol involving polymyxin B-MacConkey agar without crystal violet, PC agar, OFPVL agar, and a selective brain-heart infusion broth. Ten specimens from eight patients (8.9%) were positive by any method. The selective enrichment broth was the only medium that yielded B cepacia from all 10 positive samples, although the routine protocol was successful for eight of these. Transient carriage was identified in one patient. Epidemiological studies may be better served by the use of selective enrichment rather than selective solid media alone. Carrier status for B cepacia requires more strict definition if positive carrier status is to be accepted as having medical importance.