Scientific reports, 2016-09, Vol.6 (1), p.32442-32442, Article 32442
2016
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- Autor(en) / Beteiligte
- Titel
- A Multi-Serotype Approach Clarifies the Catabolite Control Protein A Regulon in the Major Human Pathogen Group A Streptococcus
- Ist Teil von
- Scientific reports, 2016-09, Vol.6 (1), p.32442-32442, Article 32442
- Ort / Verlag
- London: Nature Publishing Group UK
- Erscheinungsjahr
- 2016
- Quelle
- Free E-Journal (出版社公開部分のみ）
- Beschreibungen/Notizen
- Catabolite control protein A (CcpA) is a highly conserved, master regulator of carbon source utilization in gram-positive bacteria, but the CcpA regulon remains ill-defined. In this study we aimed to clarify the CcpA regulon by determining the impact of CcpA-inactivation on the virulence and transcriptome of three distinct serotypes of the major human pathogen Group A Streptococcus (GAS). CcpA-inactivation significantly decreased GAS virulence in a broad array of animal challenge models consistent with the idea that CcpA is critical to gram-positive bacterial pathogenesis. Via comparative transcriptomics, we established that the GAS CcpA core regulon is enriched for highly conserved CcpA binding motifs (i.e. cre sites). Conversely, strain-specific differences in the CcpA transcriptome seems to consist primarily of affected secondary networks. Refinement of cre site composition via analysis of the core regulon facilitated development of a modified cre consensus that shows promise for improved prediction of CcpA targets in other medically relevant gram-positive pathogens.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 2045-2322eISSN: 2045-2322DOI: 10.1038/srep32442Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5007534
- Format
- –
- Schlagworte
- 631/1647/2217/2218, 631/326/421, Animal models, Animals, Bacterial Proteins - genetics, Bacterial Proteins - metabolism, Carbon sources, Female, Gene expression, Gene Expression Profiling, Gene Expression Regulation, Bacterial, Gram-positive bacteria, Humanities and Social Sciences, Humans, Mice, multidisciplinary, Nucleotide Motifs, Pathogens, Regulon, Repressor Proteins - genetics, Repressor Proteins - metabolism, Science, Serogroup, Serotypes, Streptococcal Infections - microbiology, Streptococcal Infections - mortality, Streptococcal Infections - pathology, Streptococcus, Streptococcus infections, Streptococcus pyogenes - classification, Streptococcus pyogenes - genetics, Streptococcus pyogenes - metabolism, Streptococcus pyogenes - pathogenicity, Survival Analysis, Transcriptome, Virulence