Details

Autor(en) / Beteiligte

• Lane, Andrew N.
• Higashi, Richard M.
• Fan, Teresa W.-M.

Titel

Preclinical models for interrogating drug action in human cancers using Stable Isotope Resolved Metabolomics (SIRM)

Ist Teil von

• Metabolomics, 2016-07, Vol.12 (7), p.1, Article 118

Ort / Verlag

New York: Springer US

Erscheinungsjahr

2016

Link zum Volltext

Quelle

SpringerLink

Beschreibungen/Notizen

• Objectives In this review we compare the advantages and disadvantages of different model biological systems for determining the metabolic functions of cells in complex environments, how they may change in different disease states, and respond to therapeutic interventions. Introduction All preclinical drug-testing models have advantages and drawbacks. We compare and contrast established cell, organoid and animal models with ex vivo organ or tissue culture and in vivo human experiments in the context of metabolic readout of drug efficacy. As metabolism reports directly on the biochemical state of cells and tissues, it can be very sensitive to drugs and/or other environmental changes. This is especially so when metabolic activities are probed by stable isotope tracing methods, which can also provide detailed mechanistic information on drug action. We have developed and been applying Stable Isotope-Resolved Metabolomics to examine metabolic reprogramming of human lung cancer cells in monoculture, in mouse xenograft/explant models, and in lung cancer patients in situ (Lane et al. in Omics 15:173–182, 2011 ; Fan et al. in Metabolomics 7(2):257–269, 2011a , in Pharmacol Ther 133:366–391, 2012a , in Metabolomics 8(3):517–527, b ; Xie et al. in Cell Metab 19:795–809, 2014 ; Ren et al. in Sci Rep 4:5414, 2014 ; Sellers et al. in J Clin Investig 125(2):687–698, 2015 ). We are able to determine the influence of the tumor microenvironment using these models. We have now extended the range of models to fresh human tissue slices, similar to those originally described by Warburg (Biochem Z 142:317–333, 1923 ), which retain the native tissue architecture and heterogeneity with a paired benign versus cancer design under defined cell culture conditions. This platform offers an unprecedented human tissue model for preclinical studies on metabolic reprogramming of human cancer cells in their tissue context, and response to drug treatment (Xie et al. 2014 ). As the microenvironment of the target human tissue is retained and individual patient’s response to drugs is obtained, this platform promises to transcend current limitations of drug selection for clinical trials or treatments Conclusions Development of ex vivo human tissue and animal models with humanized organs including bone marrow and liver show considerable promise for analyzing drug responses that are more relevant to humans. Similarly using stable isotope tracer methods with these improved models in advanced stages of the drug development pipeline, in conjunction with tissue biopsy is expected significantly to reduce the high failure rate of experimental drugs in Phase II and III clinical trials.