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Early postnatal expression and localization of matrix metalloproteinases-2 and -9 during establishment of rat hippocampal synaptic circuitry
Journal of comparative neurology (1911), 2014-04, Vol.522 (6), p.1249-1263
Aujla, Paven K.
Huntley, George W.
2014
Volltextzugriff (PDF)
Details
Autor(en) / Beteiligte
Aujla, Paven K.
Huntley, George W.
Titel
Early postnatal expression and localization of matrix metalloproteinases-2 and -9 during establishment of rat hippocampal synaptic circuitry
Ist Teil von
Journal of comparative neurology (1911), 2014-04, Vol.522 (6), p.1249-1263
Ort / Verlag
United States: Blackwell Publishing Ltd
Erscheinungsjahr
2014
Quelle
Wiley-Blackwell Journals
Beschreibungen/Notizen
ABSTRACT Matrix metalloproteinases (MMPs) are extracellular proteolytic enzymes that contribute to pericellular remodeling in a variety of tissues, including brain, where they function in adult hippocampal synaptic structural and functional plasticity. Synaptic plasticity and remodeling are also important for development of connectivity, but it is unclear whether MMPs—particularly MMP‐2 and ‐9, the major MMPs operative in brain—contribute at these stages. Here, we use a combination of biochemical and anatomical methods to characterize expression and localization of MMP‐2 and MMP‐9 in early postnatal and adult rat hippocampus. Gene and protein expression of these MMPs were evident throughout hippocampus at all ages examined, but expression levels were highest during the first postnatal week. MMP‐2 and MMP‐9 immunolocalized to punctate structures within the neuropil that codistributed with foci of proteolytic activity, as well as with markers of growing axons and synapses. Taken together, discrete foci of MMP proteolysis are likely important for actively shaping and remodeling cellular and connectional architecture as hippocampal circuitry is becoming established during early postnatal life. J. Comp. Neurol. 522:1249–1263, 2014. © 2013 Wiley Periodicals, Inc. Using biochemical, immunocytochemical, and in situ zymographic methods, the authors show that in developing hippocampus, expression levels of the extracellularly acting proteases matrix metalloproteinase (MMP)‐2 and ‐9 peak during the first postnatal week. At all postnatal ages, MMP‐immunoreactive puncta codistribute with foci of proteolytic activity and with markers of growing axons and synapses. Thus, discrete foci of MMP proteolysis actively shape and remodel cellular and synaptic architecture in developing hippocampus.
Sprache
Englisch
Identifikatoren
ISSN: 0021-9967
eISSN: 1096-9861
DOI: 10.1002/cne.23468
Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4909053
Format
–
Schlagworte
Animals
,
Animals, Newborn
,
Cell Adhesion Molecules, Neuron-Glia - genetics
,
Cell Adhesion Molecules, Neuron-Glia - metabolism
,
development
,
Disks Large Homolog 4 Protein
,
extracellular proteolysis
,
Gene Expression Regulation, Developmental - physiology
,
hippocampus
,
Hippocampus - anatomy & histology
,
Hippocampus - growth & development
,
Hippocampus - metabolism
,
in situ zymography
,
Intracellular Signaling Peptides and Proteins - genetics
,
Intracellular Signaling Peptides and Proteins - metabolism
,
Matrix Metalloproteinase 2 - genetics
,
Matrix Metalloproteinase 2 - metabolism
,
Matrix Metalloproteinase 9 - genetics
,
Matrix Metalloproteinase 9 - metabolism
,
Membrane Proteins - genetics
,
Membrane Proteins - metabolism
,
Nerve Net - metabolism
,
Rats
,
Rats, Sprague-Dawley
,
RNA, Messenger - metabolism
,
Synapses - metabolism
,
synaptic plasticity
,
Synaptophysin - genetics
,
Synaptophysin - metabolism
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