Details

Autor(en) / Beteiligte

• Wiehr, Stefan
• Warnke, Philipp
• Rolle, Anna-Maria
• Schütz, Monika
• Oberhettinger, Philipp
• Kohlhofer, Ursula
• Quintanilla-Martinez, Leticia
• Maurer, Andreas
• Thornton, Christopher
• Boschetti, Frederic
• Reischl, Gerald
• Autenrieth, Ingo B
• Pichler, Bernd J
• Autenrieth, Stella E

Titel

New pathogen-specific immunoPET/MR tracer for molecular imaging of a systemic bacterial infection

Ist Teil von

• Oncotarget, 2016-03, Vol.7 (10), p.10990-11001

Ort / Verlag

United States: Impact Journals LLC

Erscheinungsjahr

2016

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• The specific and rapid detection of Enterobacteriaceae, the most frequent cause of gram-negative bacterial infections in humans, remains a major challenge. We developed a non-invasive method to rapidly detect systemic Yersinia enterocolitica infections using immunoPET (antibody-targeted positron emission tomography) with [64Cu]NODAGA-labeled Yersinia-specific polyclonal antibodies targeting the outer membrane protein YadA. In contrast to the tracer [18F]FDG, [64Cu]NODAGA-YadA uptake co-localized in a dose dependent manner with bacterial lesions of Yersinia-infected mice, as detected by magnetic resonance (MR) imaging. This was accompanied by elevated uptake of [64Cu]NODAGA-YadA in infected tissues, in ex vivo biodistribution studies, whereas reduced uptake was observed following blocking with unlabeled anti-YadA antibody. We show, for the first time, a bacteria-specific, antibody-based, in vivo imaging method for the diagnosis of a Gram-negative enterobacterial infection as a proof of concept, which may provide new insights into pathogen-host interactions.