Sie befinden Sich nicht im Netzwerk der Universität Paderborn. Der Zugriff auf elektronische Ressourcen ist gegebenenfalls nur via VPN oder Shibboleth (DFN-AAI) möglich. mehr Informationen...
Comprehensive analysis of differentially expressed rice actin depolymerizing factor gene family and heterologous overexpression of OsADF3 confers Arabidopsis Thaliana drought tolerance
Ist Teil von
Rice (New York, N.Y.), 2012-11, Vol.5 (1), p.33-33, Article 33
Ort / Verlag
New York: Springer New York
Erscheinungsjahr
2012
Quelle
EZB Electronic Journals Library
Beschreibungen/Notizen
Background
Actin depolymerizing factors (ADFs) are small actin-binding proteins. Many higher-plant ADFs has been known to involve in plant growth, development and pathogen defense. However, in rice the temporal and spatial expression of OsADF gene family and their relationship with abiotic stresses tolerance is still unknown.
Results
Here we reported the first comprehensive gene expression profile analysis of
OsADF
gene family. The
OsADF
genes showed distinct and overlapping gene expression patterns at different growth stages, tissues and abiotic stresses. We also demonstrated that both OsADF1 and OsADF3 proteins were localized in the nucleus.
OsADF1
and
OsADF3
were preferentially expressed in vascular tissues. Under ABA or abiotic stress treatments,
OsADF3
::
GUS
activity was enhanced in lateral roots and root tips. Ectopically overexpressed
OsADF3
conferred the mannitol- and drought-stress tolerance of transgenic
Arabidopsis
seedlings by increasing germination rate, primary root length and survival. Several drought-tolerance responsive genes (
RD22
,
ABF4
,
DREB2A
,
RD29A
,
PIP1
;
4
and
PIP2
;
6
) were upregulated in transgenic
Arabidopsis
under drought stress.
Conclusions
These results suggested that OsADF gene family may participate in plant abiotic stresses response or tolerance and would facilitate functional validation of other
OsADF
genes.