Details

Autor(en) / Beteiligte

• Xue, Min
• Wei, Wei
• Su, Yapeng
• Kim, Jungwoo
• Shin, Young Shik
• Mai, Wilson X
• Nathanson, David A
• Heath, James R

Titel

Chemical Methods for the Simultaneous Quantitation of Metabolites and Proteins from Single Cells

Ist Teil von

• Journal of the American Chemical Society, 2015-04, Vol.137 (12), p.4066-4069

Ort / Verlag

United States: American Chemical Society

Erscheinungsjahr

2015

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• We describe chemical approaches for integrated metabolic and proteomic assays from single cells. Quantitative assays for intracellular metabolites, including glucose uptake and three other species, are designed as surface-competitive binding assays with fluorescence readouts. This enables integration into a microarray format with functional protein immunoassays, all of which are incorporated into the microchambers of a single-cell barcode chip (SCBC). By using the SCBC, we interrogate the response of human-derived glioblastoma cancer cells to epidermal growth factor receptor inhibition. We report, for the first time, on both the intercellular metabolic heterogeneity as well as the baseline and drug-induced changes in the metabolite–phosphoprotein correlation network.