Details

Autor(en) / Beteiligte

• Li, Chang-Lin
• Li, Kai-Cheng
• Wu, Dan
• Chen, Yan
• Luo, Hao
• Zhao, Jing-Rong
• Wang, Sa-Shuang
• Sun, Ming-Ming
• Lu, Ying-Jin
• Zhong, Yan-Qing
• Hu, Xu-Ye
• Hou, Rui
• Zhou, Bei-Bei
• Bao, Lan
• Xiao, Hua-Sheng
• Zhang, Xu

Titel

Somatosensory neuron types identified by high-coverage single-cell RNA-sequencing and functional heterogeneity

Ist Teil von

• Cell research, 2016-01, Vol.26 (1), p.83-102

Ort / Verlag

England: Nature Publishing Group

Erscheinungsjahr

2016

Quelle

MEDLINE

Beschreibungen/Notizen

• Sensory neurons are distinguished by distinct signaling networks and receptive characteristics. Thus, sensory neuron types can be defined by linking transcriptome-based neuron typing with the sensory phenotypes. Here we classify somatosensory neurons of the mouse dorsal root ganglion （DRG） by high-coverage single-cell RNA-sequencing （10 950 ±1 218 genes per neuron） and neuron size-based hierarchical clustering. Moreover, single DRG neurons responding to cutaneous stimuli are recorded using an in vivo whole-cell patch clamp technique and classified by neuron-type genetic markers. Small diameter DRG neurons are classified into one type of low-threshold mechanoreceptor and five types of mechanoheat nociceptors （MHNs）. Each of the MHN types is further categorized into two subtypes. Large DRG neurons are categorized into four types, including neurexophilin 1-expressing MHNs and mechanical nociceptors （MNs） expressing BAil-associated protein 2-like I （Baiap211）. Mechanoreceptors expressing trafficking protein particle complex 3-like and Baiap211-marked MNs are subdivided into two subtypes each. These results provide a new system for cataloging somatosensory neurons and their transcriptome databases.