Details

Autor(en) / Beteiligte

• Yu, Chao
• Ji, Shu-Yan
• Dang, Yu-Jiao
• Sha, Qian-Qian
• Yuan, Yi-Feng
• Zhou, Jian-Jie
• Yan, Li-Ying
• Qiao, Jie
• Tang, Fuchou
• Fan, Heng-Yu

Titel

Oocyte-expressed yes-associated protein is a key activator of the early zygotic genome in mouse

Ist Teil von

• Cell research, 2016-03, Vol.26 (3), p.275-287

Ort / Verlag

London: Nature Publishing Group UK

Erscheinungsjahr

2016

Quelle

MEDLINE

Beschreibungen/Notizen

• In early mammalian embryos, the genome is transcriptionally quiescent until the zygotic genome activation （ZGA） which occurs 2-3 days after fertilization. Despite a long-standing effort, maternal transcription factors regulating this crucial developmental event remain largely elusive. Here, using maternal and paternal mouse models of Yapl deletion, we show that maternally accumulated yes-associated protein （YAP） in oocyte is essential for ZGA. Maternal Yapl-knoekout embryos exhibit a prolonged two-cell stage and develop into the four-cell stage at a much slower pace than the wild-type controls. Transcriptome analyses identify YAP target genes in early blastomeres; two of which, Rp113 and Rrm2, are required to mediate maternal YAP＇s effect in conferring developmental competence on preim- plantation embryos. Furthermore, the physiological YAP activator, lysophosphatidic acid, can substantially improve early development of wild-type, but not maternal Yapl-knockout embryos in both oviduct and culture. These obser- vations provide insights into the mechanisms of ZGA, and suggest potentials of YAP activators in improving the de- velopmental competence of cultured embryos in assisted human reproduction and animal biotechnology.