UNIVERSI
TÄ
TS-
BIBLIOTHEK
P
ADERBORN
Anmelden
Menü
Menü
Start
Hilfe
Blog
Weitere Dienste
Neuerwerbungslisten
Fachsystematik Bücher
Erwerbungsvorschlag
Bestellung aus dem Magazin
Fernleihe
Einstellungen
Sprache
Deutsch
Deutsch
Englisch
Farbschema
Hell
Dunkel
Automatisch
Sie befinden Sich nicht im Netzwerk der Universität Paderborn. Der Zugriff auf elektronische Ressourcen ist
gegebenenfalls
nur via VPN oder Shibboleth (DFN-AAI) möglich.
mehr Informationen...
Universitätsbibliothek
Katalog
Suche
Details
Zur Ergebnisliste
Ergebnis 12 von 368
Datensatz exportieren als...
BibTeX
Mesenchymal Deletion of Histone Demethylase NO66 in Mice Promotes Bone Formation
Journal of bone and mineral research, 2015-09, Vol.30 (9), p.1608-1617
Chen, Qin
Sinha, Krishna
Deng, Jian Min
Yasuda, Hideyo
Krahe, Ralf
Behringer, Richard R
de Crombrugghe, Benoit
2015
Details
Autor(en) / Beteiligte
Chen, Qin
Sinha, Krishna
Deng, Jian Min
Yasuda, Hideyo
Krahe, Ralf
Behringer, Richard R
de Crombrugghe, Benoit
Titel
Mesenchymal Deletion of Histone Demethylase NO66 in Mice Promotes Bone Formation
Ist Teil von
Journal of bone and mineral research, 2015-09, Vol.30 (9), p.1608-1617
Ort / Verlag
United States: Wiley Subscription Services, Inc
Erscheinungsjahr
2015
Link zum Volltext
Quelle
MEDLINE
Beschreibungen/Notizen
ABSTRACT Our previous studies indicated that the Jumonji C (JmjC)‐domain‐containing NO66 is a histone demethylase with specificity for methylated histone H3K4 and H3K36. NO66 binds to the transcription factor Osterix (Osx) and inhibits its transcriptional activity in promoter assays. However, the physiological role of NO66 in formation of mammalian bones is unknown. Here, using a genetically engineered mouse model, we show that during early skeletal development, Prx1‐Cre–dependent mesenchymal deletion of NO66 promotes osteogenesis and formation of both endochondral as well as intramembranous skeletal elements, leading to a larger skeleton and a high bone mass phenotype in adult mice. The excess bone formation in mice where NO66 was deleted in cells of mesenchymal origin is associated with an increase in the number of preosteoblasts and osteoblasts. Further analysis revealed that in the embryonic limbs and adult calvaria of mice with deletion of NO66 in cells of mesenchymal origin, expression of several genes including bone morphogenetic protein 2 (Bmp2), insulin‐like growth factor 1 (Igf1), and osteoclast inhibitor osteoprotegerin was increased, concurrent with an increase in expression of bone formation markers such as osterix (Osx), type I collagen, and bone sialoprotein (Bsp). Taken together, our results provide the first in vivo evidence that NO66 histone demethylase plays an important role in mammalian osteogenesis during early development as well as in adult bone homeostasis. We postulate that NO66 regulates bone formation, at least in part, via regulating the number of bone‐forming cells and expression of multiple genes that are critical for these processes. © 2015 American Society for Bone and Mineral Research.
Sprache
Englisch
Identifikatoren
ISSN: 0884-0431
eISSN: 1523-4681
DOI: 10.1002/jbmr.2494
Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4780322
Format
–
Schlagworte
3T3 Cells
,
Animals
,
Bone and Bones - metabolism
,
BONE FORMATION
,
Bone growth
,
Bone mass
,
Bone morphogenetic protein 2
,
Bone Morphogenetic Protein 2 - metabolism
,
Bone sialoprotein
,
Bone turnover
,
Calvaria
,
Cell Differentiation
,
Clonal deletion
,
Collagen (type I)
,
Collagen Type I - metabolism
,
DEVELOPMENT
,
Embryos
,
Female
,
Fluoresceins - chemistry
,
Gene Deletion
,
Gene Expression Regulation
,
Genetic engineering
,
Genotype
,
Histones - metabolism
,
Homeostasis
,
Insulin
,
Insulin-like growth factor I
,
Insulin-Like Growth Factor I - metabolism
,
Integrin-Binding Sialoprotein - metabolism
,
Jumonji Domain-Containing Histone Demethylases - genetics
,
Jumonji Domain-Containing Histone Demethylases - metabolism
,
KNOCKOUT MICE
,
Male
,
Mesenchyme
,
Mesoderm - metabolism
,
Mice
,
Mice, Knockout
,
NO66
,
Osteoblasts
,
Osteoblasts - metabolism
,
Osteogenesis
,
Osteogenesis - genetics
,
Osteoprotegerin
,
Osx
,
Phenotype
,
Phenotypes
,
Skeleton
,
Sp7 Transcription Factor
,
Transcription Factors - metabolism
,
X-Ray Microtomography
Weiterführende Literatur
Empfehlungen zum selben Thema automatisch vorgeschlagen von
bX