Details

Autor(en) / Beteiligte

• Cardoso, Marlon H
• Ribeiro, Suzana M
• Nolasco, Diego O
• de la Fuente-Núñez, César
• Felício, Mário R
• Gonçalves, Sónia
• Matos, Carolina O
• Liao, Luciano M
• Santos, Nuno C
• Hancock, Robert E W
• Franco, Octávio L
• Migliolo, Ludovico

Titel

A polyalanine peptide derived from polar fish with anti-infectious activities

Ist Teil von

• Scientific reports, 2016-02, Vol.6 (1), p.21385-21385, Article 21385

Ort / Verlag

England: Nature Publishing Group

Erscheinungsjahr

2016

Link zum Volltext

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• Due to the growing concern about antibiotic-resistant microbial infections, increasing support has been given to new drug discovery programs. A promising alternative to counter bacterial infections includes the antimicrobial peptides (AMPs), which have emerged as model molecules for rational design strategies. Here we focused on the study of Pa-MAP 1.9, a rationally designed AMP derived from the polar fish Pleuronectes americanus. Pa-MAP 1.9 was active against Gram-negative planktonic bacteria and biofilms, without being cytotoxic to mammalian cells. By using AFM, leakage assays, CD spectroscopy and in silico tools, we found that Pa-MAP 1.9 may be acting both on intracellular targets and on the bacterial surface, also being more efficient at interacting with anionic LUVs mimicking Gram-negative bacterial surface, where this peptide adopts α-helical conformations, than cholesterol-enriched LUVs mimicking mammalian cells. Thus, as bacteria present varied physiological features that favor antibiotic-resistance, Pa-MAP 1.9 could be a promising candidate in the development of tools against infections caused by pathogenic bacteria.