Details

Autor(en) / Beteiligte

• Zhang, Yang
• Luo, Jian-Xing
• Hu, Xiao-Yu
• Yang, Fang
• Zhong, Sen
• Lin, Wu

Titel

Improved prescription of taohechengqi-tang alleviates D-galactosamine acute liver failure in rats

Ist Teil von

• World journal of gastroenterology : WJG, 2016-02, Vol.22 (8), p.2558-2565

Ort / Verlag

United States: Baishideng Publishing Group Inc

Erscheinungsjahr

2016

Quelle

EZB-FREE-00999 freely available EZB journals

Beschreibungen/Notizen

• AIM: To investigate the hepatoprotective effect of improved prescription of Taohechengqi-tang(IPTT) against acute liver failure(ALF) in rats.METHODS: Seventy specific pathogen free male Wistar rats were randomly divided into four groups: control group(normal rats, n = 10), ALF group(ALF model,n=20), Stronger Neo-Minophagen C(SNMC) group(ALF model + SNMC, n = 20), and IPTT group(ALF model + IPTT, n = 20). The ALF model group was administered an intraperitoneal injection of D-galactosamine(1.4 g/kg), and the control group received normal saline intraperitoneally. The SNMC and IPTT groups were treated with SMMC(15.6 mg/kg) or IPTT(28.6 g/kg) by gavage at 24 h intervals, and the ALF and control groups were treated with normal saline. At 36 h after injection, serum alanine aminotransferase, aspartate aminotransferase, total bilirubin, albumin, and cholinesterase and prothrombintime were determined, and liver histopathological scores were observed by microscopy after hematoxylin and eosin staining. m RNA expression of high mobility group box(HMGB) 1, toll-like receptor(TLR) 4, nuclear factor kappa B(NF-κB) and caspase-3 were analyzed via fluorescence quantitative reverse transcriptase polymerase chain reaction. Proliferating cell nuclear antigen(PCNA) immunohistochemistry in liver tissue was also performed.RESULTS: D-galactosamine notably decreased the biochemical and coagulation profiles in serum. IPTT not only improved liver function and histopathology but also normalized the gene expression levels in liver tissue. Compared with the model group, in the IPTT and SNMC groups, HMGB1 m RNA/β-actin(0.06 ± 0.03, 0.11 ± 0.04 vs 0.25 ± 0.04, P < 0.05); TLR4 m RNA/β-actin(0.07 ± 0.02, 0.22 ± 0.08 vs 0.41 ± 0.22, P < 0.05); NF-κB m RNA/β-actin(0.74 ± 0.41, 1.78 ± 0.64 vs 2.68 ± 1.35, P < 0.05); and caspase-3 m RNA/β-actin levels were all significantly reduced(1.61 ± 0.45, 2.57 ± 1.04 vs 3.41 ± 0.85, P < 0.05). The gene expression levels were significantly lower in the IPTT group than in the SNMC group(P < 0.05). Compared with the model group, the PCNA expression in liver tissue was significantly enhanced in the IPTT and SNMC groups(36.34 ± 4.91, 25.57 ± 2.94 vs 17.55 ± 2.40, P < 0.05). CONCLUSION: IPTT attenuates inflammation in ALF via inhibition of HMGB1 production, which may contribute to limited liver regeneration.