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Scientific reports, 2016-02, Vol.6 (1), p.20315, Article 20315
2016
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Autor(en) / Beteiligte
Titel
Exploiting the CRISPR/Cas9 System for Targeted Genome Mutagenesis in Petunia
Ist Teil von
  • Scientific reports, 2016-02, Vol.6 (1), p.20315, Article 20315
Ort / Verlag
England: Nature Publishing Group
Erscheinungsjahr
2016
Quelle
Free E-Journal (出版社公開部分のみ)
Beschreibungen/Notizen
  • Recently, CRISPR/Cas9 technology has emerged as a powerful approach for targeted genome modification in eukaryotic organisms from yeast to human cell lines. Its successful application in several plant species promises enormous potential for basic and applied plant research. However, extensive studies are still needed to assess this system in other important plant species, to broaden its fields of application and to improve methods. Here we showed that the CRISPR/Cas9 system is efficient in petunia (Petunia hybrid), an important ornamental plant and a model for comparative research. When PDS was used as target gene, transgenic shoot lines with albino phenotype accounted for 55.6%-87.5% of the total regenerated T0 Basta-resistant lines. A homozygous deletion close to 1 kb in length can be readily generated and identified in the first generation. A sequential transformation strategy--introducing Cas9 and sgRNA expression cassettes sequentially into petunia--can be used to make targeted mutations with short indels or chromosomal fragment deletions. Our results present a new plant species amenable to CRIPR/Cas9 technology and provide an alternative procedure for its exploitation.
Sprache
Englisch
Identifikatoren
ISSN: 2045-2322
eISSN: 2045-2322
DOI: 10.1038/srep20315
Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4738242

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