ASN neuro, 2015-01, Vol.7 (1), p.175909141456687
2015
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- Autor(en) / Beteiligte
- Titel
- Brain-Derived Neurotrophic Factor Deficiency Restricts Proliferation of Oligodendrocyte Progenitors Following Cuprizone-Induced Demyelination
- Ist Teil von
- ASN neuro, 2015-01, Vol.7 (1), p.175909141456687
- Ort / Verlag
- Los Angeles, CA: SAGE Publications
- Erscheinungsjahr
- 2015
- Quelle
- Free E-Journal (出版社公開部分のみ）
- Beschreibungen/Notizen
- Brain-derived neurotrophic factor (BDNF) is a member of the neurotrophin family of growth factors that through its neurotrophic tyrosine kinase, receptor, type 2 (TrkB) receptor, increases 5-bromo-2-deoxyuridine incorporation in oligodendrocyte progenitor cells (OPCs) in culture. Roles in vivo are less well understood; however, increases in numbers of OPCs are restricted in BDNF+/− mice following cuprizone-elicited demyelination. Here, we investigate whether these blunted increases in OPCs are associated with changes in proliferation. BDNF+/+ and BDNF+/− mice were fed cuprizone-containing or control feed. To assess effects on OPC numbers, platelet-derived growth factor receptor alpha (PDGFRα)+ or NG2+ cells were counted. To monitor DNA synthesis, 5-ethynyl-2′-deoxyuridine (EdU) was injected intraperitoneally and colocalized with PDGFRα+ cells. Alternatively, proliferating cell nuclear antigen (PCNA) was colocalized with PDGFRα or NG2. Labeling indices were determined in the BDNF+/+ and BDNF+/− animals. After 4 or 5 weeks of control feed, BDNF+/− mice exhibit similar numbers of OPCs compared with BDNF+/+ animals. The labeling indices for EdU and PCNA also were not significantly different, suggesting that neither the DNA synthesis phase (S phase) nor the proliferative pool size was different between genotypes. In contrast, when mice were challenged by cuprizone for 4 or 5 weeks, increases in OPCs observed in BDNF+/+ mice were reduced in the BDNF+/− mice. This difference in elevations in cell number was accompanied by decreases in EdU labeling and PCNA labeling without changes in cell death, indicating a reduction in the DNA synthesis and the proliferative pool. Therefore, levels of BDNF influence the proliferation of OPCs resulting from a demyelinating lesion.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1759-0914eISSN: 1759-0914DOI: 10.1177/1759091414566878Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4720179
- Format
- –
- Schlagworte
- Animals, Antigens - metabolism, Brain-Derived Neurotrophic Factor - deficiency, Brain-Derived Neurotrophic Factor - genetics, Bromodeoxyuridine - metabolism, Cell Proliferation - drug effects, Cell Proliferation - genetics, Cuprizone - toxicity, Demyelinating Diseases - chemically induced, Demyelinating Diseases - pathology, Disease Models, Animal, Gene Expression Regulation - drug effects, Gene Expression Regulation - genetics, In Situ Nick-End Labeling, Mice, Mice, Inbred C57BL, Mice, Transgenic, Monoamine Oxidase Inhibitors - toxicity, Oligodendroglia - physiology, Original, Phenylurea Compounds, Proliferating Cell Nuclear Antigen - metabolism, Proteoglycans - metabolism, Receptor, Platelet-Derived Growth Factor alpha - metabolism, Stem Cells - drug effects, Stem Cells - metabolism, Time Factors