Details

Autor(en) / Beteiligte

• Cacas, Jean-Luc
• Buré, Corinne
• Grosjean, Kevin
• Gerbeau-Pissot, Patricia
• Lherminier, Jeannine
• Rombouts, Yoann
• Maes, Emmanuel
• Bossard, Claire
• Gronnier, Julien
• Furt, Fabienne
• Fouillen, Laetitia
• Germain, Véronique
• Bayer, Emmanuelle
• Cluzet, Stéphanie
• Robert, Franck
• Schmitter, Jean-Marie
• Deleu, Magali
• Lins, Laurence
• Simon-Plas, Françoise
• Mongrand, Sébastien

Titel

Revisiting Plant Plasma Membrane Lipids in Tobacco: A Focus on Sphingolipids1

Ist Teil von

• Plant physiology (Bethesda), 2015-10, Vol.170 (1), p.367-384

Ort / Verlag

American Society of Plant Biologists

Erscheinungsjahr

2015

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• Glycosyl-Inositol-Phosphoryl-Ceramides are major lipids of the plasma membrane and form rafts in the outer leaflet, yet extend into the inner leaflet of the membrane through very long-chain fatty acids to interdigitate across the membrane. The lipid composition of plasma membrane ( PM ) and the corresponding detergent-insoluble membrane ( DIM ) fraction were analyzed with a specific focus on highly polar sphingolipids, so-called glycosyl inositol phosphorylceramides ( GIPC s). Using tobacco ( Nicotiana tabacum ) ‘Bright Yellow 2’ cell suspension and leaves, evidence is provided that GIPC s represent up to 40 mol % of the PM lipids. Comparative analysis of DIM s with the PM showed an enrichment of 2-hydroxylated very-long-chain fatty acid-containing GIPC s and polyglycosylated GIPC s in the DIM s. Purified antibodies raised against these GIPC s were further used for immunogold-electron microscopy strategy, revealing the distribution of polyglycosylated GIPC s in domains of 35 ± 7 nm in the plane of the PM . Biophysical studies also showed strong interactions between GIPC s and sterols and suggested a role for very-long-chain fatty acids in the interdigitation between the two PM -composing monolayers. The ins and outs of lipid asymmetry, raft formation, and interdigitation in plant membrane biology are finally discussed.