Details

Autor(en) / Beteiligte

• Tavaré, Richard
• Escuin-Ordinas, Helena
• Mok, Stephen
• McCracken, Melissa N
• Zettlitz, Kirstin A
• Salazar, Felix B
• Witte, Owen N
• Ribas, Antoni
• Wu, Anna M

Titel

An Effective Immuno-PET Imaging Method to Monitor CD8-Dependent Responses to Immunotherapy

Ist Teil von

• Cancer research (Chicago, Ill.), 2016-01, Vol.76 (1), p.73-82

Ort / Verlag

United States

Erscheinungsjahr

2016

Quelle

MEDLINE

Beschreibungen/Notizen

• The rapidly advancing field of cancer immunotherapy is currently limited by the scarcity of noninvasive and quantitative technologies capable of monitoring the presence and abundance of CD8(+) T cells and other immune cell subsets. In this study, we describe the generation of (89)Zr-desferrioxamine-labeled anti-CD8 cys-diabody ((89)Zr-malDFO-169 cDb) for noninvasive immuno-PET tracking of endogenous CD8(+) T cells. We demonstrate that anti-CD8 immuno-PET is a sensitive tool for detecting changes in systemic and tumor-infiltrating CD8 expression in preclinical syngeneic tumor immunotherapy models including antigen-specific adoptive T-cell transfer, agonistic antibody therapy (anti-CD137/4-1BB), and checkpoint blockade antibody therapy (anti-PD-L1). The ability of anti-CD8 immuno-PET to provide whole body information regarding therapy-induced alterations of this dynamic T-cell population provides new opportunities to evaluate antitumor immune responses of immunotherapies currently being evaluated in the clinic.