Scientific reports, 2016-01, Vol.6 (1), p.18430-18430, Article 18430
2016
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- Autor(en) / Beteiligte
- Titel
- Distinct effects of β1 integrin on cell proliferation and cellular signaling in MDA-MB-231 breast cancer cells
- Ist Teil von
- Scientific reports, 2016-01, Vol.6 (1), p.18430-18430, Article 18430
- Ort / Verlag
- London: Nature Publishing Group UK
- Erscheinungsjahr
- 2016
- Quelle
- Free E-Journal (出版社公開部分のみ）
- Beschreibungen/Notizen
- An aberrant expression of integrin β1 has been implicated in breast cancer progression. Here, we compared the cell behaviors of wild-type (WT), β1 gene deleted (KO) and β1 gene restored (Res) MDA-MB-231 cells. Surprisingly, the expression of β1 exhibited opposite effects on cell proliferation. These effects were dependent on cell densities and they showed an up-regulation of cell proliferation when cells were cultured under sparse conditions and a down-regulation of cell growth under dense conditions. By comparison with WT cells, the phosphorylation levels of ERK in KO cells were consistently suppressed under sparse culture conditions, but consistently up-regulated under dense culture conditions. The phosphorylation levels of EGFR were increased in the KO cells. By contrast, the phosphorylation levels of AKT were decreased in the KO cells. The abilities for both colony and tumor formation were significantly suppressed in the KO cells, suggesting that β1 plays an important role in cell survival signaling for tumorigenesis. These aberrant phenotypes in the KO cells were rescued in the Res cells. Taken together, these results clearly showed the distinct roles of β1 in cancer cells: the inhibition of cell growth and the promotion of cell survival, which may shed light on cancer therapies.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 2045-2322eISSN: 2045-2322DOI: 10.1038/srep18430Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4700444
- Format
- –
- Schlagworte
- 13, 13/31, 13/51, 13/95, 42, 42/109, 42/41, 631/80/79/1236, 631/80/86/2368, 82, 82/29, Breast Neoplasms - genetics, Breast Neoplasms - metabolism, Breast Neoplasms - pathology, Cell Adhesion - genetics, Cell Line, Tumor, Cell Movement - genetics, Cell Proliferation - drug effects, Cell Transformation, Neoplastic - genetics, Female, Gene Deletion, Gene Expression, Gene Knockout Techniques, Humanities and Social Sciences, Humans, Integrin beta1 - chemistry, Integrin beta1 - genetics, Integrin beta1 - metabolism, multidisciplinary, Protein Kinase Inhibitors, Protein Subunits - genetics, Quinazolines - pharmacology, Receptor, Epidermal Growth Factor - antagonists & inhibitors, Receptor, Epidermal Growth Factor - metabolism, Science, Signal Transduction, Tyrphostins - pharmacology