Virology (New York, N.Y.), 2016-01, Vol.487, p.249-259
2016
Volltextzugriff (PDF)
Details
- Autor(en) / Beteiligte
- Titel
- Combined therapy of oncolytic adenovirus and temozolomide enhances lung cancer virotherapy in vitro and in vivo
- Ist Teil von
- Virology (New York, N.Y.), 2016-01, Vol.487, p.249-259
- Ort / Verlag
- United States: Elsevier Inc
- Erscheinungsjahr
- 2016
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Abstract Oncolytic adenoviruses (OAds) are very promising for the treatment of lung cancer. However, OAd-based monotherapeutics have not been effective during clinical trials. Therefore, the effectiveness of virotherapy must be enhanced by combining OAds with other therapies. In this study, the therapeutic potential of OAd in combination with temozolomide (TMZ) was evaluated in lung cancer cells in vitro and in vivo . The combination of OAd and TMZ therapy synergistically enhanced cancer cell death; this enhanced cancer cell death may be explained via three related mechanisms: apoptosis, virus replication, and autophagy. Autophagy inhibition partially protected cancer cells from this combined therapy. This combination significantly suppressed the growth of subcutaneous H441 lung cancer xenograft tumors in athymic nude mice. In this study, we have provided an experimental rationale to test OAds in combination with TMZ in a lung cancer clinical trial.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0042-6822eISSN: 1096-0341DOI: 10.1016/j.virol.2015.10.019Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4679495
- Format
- –
- Schlagworte
- Adenoviridae - physiology, Adenoviridae Infections - virology, Adenovirus, Adenovirus E1B Proteins - genetics, Animals, Antineoplastic Agents, Alkylating - therapeutic use, Apoptosis, Apoptosis - drug effects, Autophagy, Autophagy - drug effects, Cancer, Cell Line, Tumor, Combined Modality Therapy - methods, Dacarbazine - analogs & derivatives, Dacarbazine - therapeutic use, DNA Modification Methylases - metabolism, DNA Repair Enzymes - metabolism, HEK293 Cells, Humans, Infectious Disease, JNK Mitogen-Activated Protein Kinases - genetics, Lung, Lung Neoplasms - therapy, Male, Mice, Mice, Inbred BALB C, Mice, Nude, Oncolytic, Oncolytic Virotherapy - methods, Oncolytic Viruses - physiology, Temozolomide, Tumor Suppressor Proteins - metabolism, Virus Replication, Xenograft Model Antitumor Assays - methods