Details

Autor(en) / Beteiligte

• Sen, Nandini
• Mukherjee, Gourab
• Arvin, Ann M.

Titel

Single cell mass cytometry reveals remodeling of human T cell phenotypes by varicella zoster virus

Ist Teil von

• Methods (San Diego, Calif.), 2015-11, Vol.90, p.85-94

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2015

Quelle

MEDLINE

Beschreibungen/Notizen

• •Deep protein profiling of virus infected cells by single cell mass cytometry.•Single cell analysis of virus interactions with heterogeneous host cells.•VZV activates T cell signaling and remodels surface proteins regardless of baseline.•Statistical tools to analyze virus mediated changes using mass cytometry data.•Single cell linkage density estimation (SLIDE) quantifies T cell remodeling by VZV. The recent application of mass cytometry (CyTOF) to biology provides a ‘systems’ approach to monitor concurrent changes in multiple host cell factors at the single cell level. We used CyTOF to evaluate T cells infected with varicella zoster virus (VZV) infection, documenting virus-mediated phenotypic and functional changes caused by this T cell tropic human herpesvirus. Here we summarize our findings using two complementary panels of antibodies against surface and intracellular signaling proteins to elucidate the consequences of VZV-mediated perturbations on the surface and in signaling networks of infected T cells. CyTOF data was analyzed by several statistical, analytical and visualization tools including hierarchical clustering, orthogonal scaling, SPADE, viSNE, and SLIDE. Data from the mass cytometry studies demonstrated that VZV infection led to ‘remodeling’ of the surface architecture of T cells, promoting skin trafficking phenotypes and associated with concomitant activation of T-cell receptor and PI3-kinase pathways. This method offers a novel approach for understanding viral interactions with differentiated host cells important for pathogenesis.