Details

Autor(en) / Beteiligte

• Xu, Huanbin
• Wang, Xiaolei
• Lackner, Andrew A.
• Veazey, Ronald S.

Titel

Type 3 innate lymphoid cell depletion is mediated by TLRs in lymphoid tissues of simian immunodeficiency virus‐infected macaques

Ist Teil von

• The FASEB journal, 2015-12, Vol.29 (12), p.5072-5080

Ort / Verlag

Bethesda, MD, USA: Federation of American Societies for Experimental Biology

Erscheinungsjahr

2015

Link zum Volltext

Quelle

Wiley Blackwell Single Titles

Beschreibungen/Notizen

• ABSTRACT Innate lymphoid cells (ILCs) type 3, also known as lymphoid tissue inducer cells, plays a major role in both the development and remodeling of organized lymphoid tissues and the maintenance of adaptive immune responses. HIV/simian immunodeficiency virus (SIV) infection causes breakdown of intestinal barriers resulting in microbial translocation, leading to systemic immune activation and disease progression. However, the effects of HIV/SIV infection on ILC3 are unknown. Here, we analyzed ILC3 from mucosal and systemic lymphoid tissues in chronically SIV‐infected macaques and uninfected controls. ILC3 cells were defined and identified in macaque lymphoid tissues as non‐T, non‐B (lineage‐negative), c‐Kit+IL‐7Rα+ (CD117+CD127+) cells. These ILC3 cells highly expressed CD90 (∼63%) and aryl hydrocarbon receptor and produced IL‐17 (∼63%), IL‐22 (∼36%), and TNF‐α (∼72%) but did not coexpress CD4 or NK cell markers. The intestinal ILC3 cell loss correlated with the reduction of total CD4+ T cells and T helper (Th) 17 and Th22 cells in the gut during SIV infection (P< 0.001). Notably, ILC3 could be induced to undergo apoptosis by microbial products through the TLR2 (lipoteichoic acid) and/or TLR4 (LPS) pathway. These findings indicated that persistent microbial translocation may result in loss of ILC3 in lymphoid tissues in SIV‐infected macaques, further contributing to the HIV‐induced impairment of gut‐associated lymphoid tissue structure and function, especially in mucosal tissues.—Xu, H., Wang, X., Lackner, A. A., Veazey, R. S. Type 3 innate lymphoid cell depletion is mediated by TLRs in lymphoid tissues of simian immunodeficiency virus‐infected macaques. FASEB J. 29, 5072–5080 (2015). www.fasebj.org