Acta pharmacologica Sinica, 2014-03, Vol.35 (3), p.356-362
2014
Details
- Autor(en) / Beteiligte
- Titel
- The effect of inhibition of endoplasmic reticulum stress on lipolysis in white adipose tissue in a rat model of chronic kidney disease
- Ist Teil von
- Acta pharmacologica Sinica, 2014-03, Vol.35 (3), p.356-362
- Ort / Verlag
- United States: Nature Publishing Group
- Erscheinungsjahr
- 2014
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Aim; Lipolysis in fat tissue plays an important role in the development of metabolic disturbances, a characteristic feature of chronic kidney disease （CKD）. In the present study, we tested the hypothesis that the inhibition of endoplasmic reticulum （ER） stress could alleviate lipolysis in white adipose tissue in a rat model of CKD. Methods： A rat model of CKD was established by a method of reduced renal mass （RRM）. Lipolysis was measured as the release of glycerol in ex vivo fat pads and cultured primary adipocytes. The activity of lipases and markers of ER stress were measured by Western blotting and immunoprecipitation. Results; Our data showed that lipolysis in visceral white adipose tissue was increased in RRM rats compared with control rats. In addition, increased phosphorylation of hormone-sensitive lipase （HSL） and binding of adipose triglyceride lipase （ATGL） to comparative gene identification-58 （CGI-58） protein were observed in the RRM rats. The phosphorylation of ER stress markers, including IREla, PERK, and eukaryotic initiation factor （elF） 2a, and the expression of ER stress marker 78 kDa glucose-regulated protein （GRP78） were significantly increased in RRM rats. Treatment with an inhibitor of ER stress partially but significantly alleviated lipolysis, and this alleviation was accompanied by reduced binding of ATGL to CGI-58. Conclusion： Our results showed that enhanced lipolysis and ER stress occurred in visceral white adipose tissue in a rat model of CKD. Moreover, inhibition of ER stress significantly alleviated lipolysis. These findings suggest that ER stress is a potential therapeutic target for the metabolic disturbances associated with CKD.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1671-4083eISSN: 1745-7254DOI: 10.1038/aps.2013.177Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4647896
- Format
- –
- Schlagworte
- Acyltransferases - metabolism, Adipocytes - drug effects, Adipocytes - metabolism, Animals, Body fat, Cells, Cultured, Disease Models, Animal, eIF-2 Kinase - metabolism, Endoplasmic Reticulum - drug effects, Endoplasmic Reticulum - metabolism, Endoplasmic Reticulum Stress - drug effects, Eukaryotic Initiation Factor-2 - metabolism, Glycerol - metabolism, Intra-Abdominal Fat - drug effects, Intra-Abdominal Fat - metabolism, Lipase - metabolism, Lipolysis - drug effects, Male, Membrane Proteins - metabolism, Original, Phenylbutyrates - pharmacology, Phosphorylation, Protein-Serine-Threonine Kinases - metabolism, Rats, Sprague-Dawley, Renal Insufficiency, Chronic - metabolism, Signal Transduction - drug effects, Sterol Esterase - metabolism, 代谢紊乱, 内质网应激, 大鼠模型, 慢性, 激素敏感脂肪酶, 肾脏疾病, 脂肪组织, 脂解