Details

Autor(en) / Beteiligte

• Zeng, Zhenshun
• Guo, Xing-Pan
• Li, Baiyuan
• Wang, Pengxia
• Cai, Xingsheng
• Tian, Xinpeng
• Zhang, Si
• Yang, Jin-Long
• Wang, Xiaoxue

Titel

Characterization of self-generated variants in Pseudoalteromonas lipolytica biofilm with increased antifouling activities

Ist Teil von

• Applied microbiology and biotechnology, 2015-12, Vol.99 (23), p.10127-10139

Ort / Verlag

Berlin/Heidelberg: Springer Berlin Heidelberg

Erscheinungsjahr

2015

Quelle

SpringerLink

Beschreibungen/Notizen

• Pseudoalteromonas is widespread in various marine environments, and most strains can affect invertebrate larval settlement and metamorphosis by forming biofilms. However, the impact and the molecular basis of population diversification occurring in Pseudoalteromonas biofilms are poorly understood. Here, we show that morphological diversification is prevalent in Pseudoalteromonas species during biofilm formation. Two types of genetic variants, wrinkled (frequency of 12 ± 5 %) and translucent (frequency of 5 ± 3 %), were found in Pseudoalteromonas lipolytica biofilms. The inducing activities of biofilms formed by the two variants on larval settlement and metamorphosis of the mussel Mytilus coruscus were significantly decreased, suggesting strong antifouling activities. Using whole-genome re-sequencing combined with genetic manipulation, two genes were identified to be responsible for the morphology alternations. A nonsense mutation in AT00 _ 08765 led to a wrinkled morphology due to the overproduction of cellulose, whereas a point mutation in AT00 _ 17125 led to a translucent morphology via a reduction in capsular polysaccharide production. Taken together, the results suggest that the microbial behavior on larval settlement and metamorphosis in marine environment could be affected by the self-generated variants generated during the formation of marine biofilms, thereby rendering potential application in biocontrol of marine biofouling.