Details

Autor(en) / Beteiligte

• Pires, Alause da Silva
• Borges, Arissa Felipe
• Cappellazzo Coelho, Adriano
• Dorta, Miriam Leandro
• Lino Junior, Ruy de Souza
• Pereira, Ledice Inacia de Araújo
• Pinto, Sebastião Alves
• Pelli de Oliveira, Milton Adriano
• de Matos, Grazzielle Guimarães
• Abrahamsohn, Ises A.
• Uliana, Silvia Reni B.
• Lima, Glória Maria Collet de Araújo
• Ribeiro-Dias, Fátima
• Chomicz, Lidia

Titel

Identification and Biological Characterization of Leishmania (Viannia) guyanensis Isolated from a Patient with Tegumentary Leishmaniasis in Goiás, a Nonendemic Area for This Species in Brazil

Ist Teil von

• BioMed research international, 2015-01, Vol.2015, p.350764-11

Ort / Verlag

United States: Hindawi Publishing Corporation

Erscheinungsjahr

2015

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• The aim of this study was to characterize clinical field isolates of Leishmania spp. obtained from patients with American Tegumentary Leishmaniasis (ATL) who live in Goiás state, Brazil. The presumed areas of infection were in Goiás, Tocantins, and Pará states. Three isolates of parasites were identified as L. (Viannia) braziliensis and one as L. (V.) guyanensis. The in vitro growth profiles were found to be similar for all parasites. Nevertheless, in C57BL/6 mice, L. (V.) guyanensis infection was better controlled than L. (V.) braziliensis. Yet in C57BL/6 mice deficient in interferon gamma, L. (V.) guyanensis lesions developed faster than those caused by L. (V.) braziliensis isolates. In BALB/c mice, the development of lesions was similar for isolates from both species; however, on the 11th week of infection, amastigotes could not be observed in macrophages from L. (V.) guyanensis-infected mice. Thus, L. (V.) guyanensis can be circulating in Goiás, a state where autochthonous cases of this species had not yet been reported. Considering the difficulties to differentiate L. (V.) guyanensis from L. (V.) braziliensis at the molecular, morphological, and clinical (human and murine models) levels, the presence of L. (V.) guyanensis infections is possibly underestimated in several regions of Brazil.