Details
- Autor(en) / Beteiligte
- Titel
- Multi-Modal Imaging with a Toolbox of Influenza A Reporter Viruses
- Ist Teil von
- Viruses, 2015-10, Vol.7 (10), p.5319-5327
- Ort / Verlag
- Switzerland: MDPI
- Erscheinungsjahr
- 2015
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Reporter viruses are useful probes for studying multiple stages of the viral life cycle. Here we describe an expanded toolbox of fluorescent and bioluminescent influenza A reporter viruses. The enhanced utility of these tools enabled kinetic studies of viral attachment, infection, and co-infection. Multi-modal bioluminescence and positron emission tomography-computed tomography (PET/CT) imaging of infected animals revealed that antiviral treatment reduced viral load, dissemination, and inflammation. These new technologies and applications will dramatically accelerate in vitro and in vivo influenza virus studies.
- Sprache
- Englisch
- Identifikatoren
- eISSN: 1999-4915DOI: 10.3390/v7102873Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4632381
- Format
- –
- Schlagworte
- Animals, Antiviral Agents - therapeutic use, Communication, Disease Models, Animal, Female, Genes, Reporter, Influenza A virus - growth & development, Luminescent Measurements - methods, Mice, Inbred BALB C, Multimodal Imaging - methods, Optical Imaging - methods, Orthomyxoviridae Infections - drug therapy, Orthomyxoviridae Infections - pathology, Orthomyxoviridae Infections - virology, Staining and Labeling - methods, Treatment Outcome, Viral Load, Whole Body Imaging - methods