Cell cycle (Georgetown, Tex.), 2015-01, Vol.14 (12), p.1925-1931
2015
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Details
- Autor(en) / Beteiligte
- Titel
- The microtubule nucleation activity of centrobin in both the centrosome and cytoplasm
- Ist Teil von
- Cell cycle (Georgetown, Tex.), 2015-01, Vol.14 (12), p.1925-1931
- Ort / Verlag
- United States: Taylor & Francis
- Erscheinungsjahr
- 2015
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Centrobin resides in daughter centriole and play a critical role in centriole duplication. Nucleation and stabilization of microtubules are known biological activities of centrobin. Here, we report a specific localization of centrobin outside the centrosome. Centrobin was associated with the stable microtubules. In hippocampal cells, centrobin formed cytoplasmic dots in addition to the localization at both centrosomes with the mother and daughter centrioles. Such specific localization pattern suggests that cytoplasmic centrobin is not just a reserved pool for centrosomal localization but also has a specific role in the cytoplasm. In fact, centrobin enhanced microtubule formation outside as well as inside the centrosome. These results propose specific roles of the cytoplasmic centrobin for noncentrosomal microtubule formation in specific cell types and during the cell cycle.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1538-4101eISSN: 1551-4005DOI: 10.1080/15384101.2015.1041683Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4614417
- Format
- –
- Schlagworte
- Animals, Cell Cycle, Cell Cycle Proteins - physiology, Cell Line, Cell Line, Tumor, Centrioles - metabolism, Centrosome - metabolism, Cytoplasm - metabolism, Green Fluorescent Proteins - metabolism, Hippocampus - metabolism, Humans, Mice, Microtubules - metabolism, NIMA-Related Kinases, Phosphorylation, Protein Structure, Tertiary, Protein-Serine-Threonine Kinases - metabolism, RNA Interference, RNA, Small Interfering - metabolism, Transfection