Journal of Zhejiang University. B. Science, 2015-10, Vol.16 (10), p.824-831
2015
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Details
- Autor(en) / Beteiligte
- Titel
- Generation of insect-resistant and glyphosate-tolerant rice by introduction of a T-DNA containing two Bt insecticidal genes and an EPSPS gene
- Ist Teil von
- Journal of Zhejiang University. B. Science, 2015-10, Vol.16 (10), p.824-831
- Ort / Verlag
- Hangzhou: Zhejiang University Press
- Erscheinungsjahr
- 2015
- Quelle
- SpringerLink
- Beschreibungen/Notizen
- Insect resistance and glyphosate tolerance have been two of the most important traits in the genetic improvement of various crops. In this study, two Bacillus thuringiensis (Bt) insecticidal genes, Cry1Ac and Cry1Ig , and a modified glyphosate-tolerant 5-enolpyruvylshikimate-3-phosphate synthase ( EPSPS ) gene ( G10 ) were combined into a single transferred DNA (T-DNA) fragment and introduced into rice by Agrobacterium -mediated transformation. A transgenic line with single-copy T-DNA insertion named GAI-14 was found to be highly resistant to striped stem borer and rice leaf roller, and tolerant to glyphosate. Analysis of T-DNA border sequence suggested that the transgenes were inserted at the chromosome 3 and appeared to have not interrupted any known or putative genes. A field trial observed no significant difference in the basic agronomic traits between GAI-14 and the recipient rice.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1673-1581eISSN: 1862-1783DOI: 10.1631/jzus.B1500056Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4609534
- Format
- –
- Schlagworte
- 3-Phosphoshikimate 1-Carboxyvinyltransferase - genetics, 3-Phosphoshikimate 1-Carboxyvinyltransferase - metabolism, Agronomy, Animals, Bacillus thuringiensis, Bacillus thuringiensis Toxins, Bacterial Proteins - genetics, Bacterial Proteins - metabolism, Biomedical and Life Sciences, Biomedicine, Borers, Chromosome 3, Crops, Cry1Ac toxin, Deoxyribonucleic acid, DNA, DNA, Bacterial - genetics, Drug Tolerance, Endotoxins - genetics, Endotoxins - metabolism, EPSPS gene, Fragmentation, Genes, Genetic Enhancement - methods, Genetic improvement, Genetic transformation, Glycine - administration & dosage, Glycine - analogs & derivatives, Glyphosate, Hemolysin Proteins - genetics, Hemolysin Proteins - metabolism, Insecta - physiology, Insecticide resistance, Insecticides - metabolism, Insertion, Nucleotide sequence, Oryza - drug effects, Oryza - parasitology, Oryza - physiology, Pest Control, Biological - methods, Phosphate, Phosphates, Plants, Genetically Modified - drug effects, Plants, Genetically Modified - parasitology, Plants, Genetically Modified - physiology, Rice, T-DNA, Transcription, Transfection - methods, Transgenes