Details

Autor(en) / Beteiligte

• Cisek, Richard
• Tokarz, Danielle
• Steup, Martin
• Tetlow, Ian J
• Emes, Michael J
• Hebelstrup, Kim H
• Blennow, Andreas
• Barzda, Virginijus

Titel

Second harmonic generation microscopy investigation of the crystalline ultrastructure of three barley starch lines affected by hydration

Ist Teil von

• Biomedical optics express, 2015-10, Vol.6 (10), p.3694-3700

Ort / Verlag

United States: Optical Society of America

Erscheinungsjahr

2015

Quelle

EZB Free E-Journals

Beschreibungen/Notizen

• Second harmonic generation (SHG) microscopy is employed to study changes in crystalline organization due to altered gene expression and hydration in barley starch granules. SHG intensity and susceptibility ratio values (R'SHG ) are obtained using reduced Stokes-Mueller polarimetric microscopy. The maximum R'SHG values occur at moderate moisture indicating the narrowest orientation distribution of nonlinear dipoles from the cylindrical axis of glucan helices. The maximum SHG intensity occurs at the highest moisture and amylopectin content. These results support the hypothesis that SHG is caused by ordered hydrogen and hydroxyl bond networks which increase with hydration of starch granules.