Details

Autor(en) / Beteiligte

• Ayre, D. Craig
• Elstner, Marcus
• Smith, Nicole C.
• Moores, Emily S.
• Hogan, Andrew M.
• Christian, Sherri L.

Titel

Dynamic regulation of CD24 expression and release of CD24‐containing microvesicles in immature B cells in response to CD24 engagement

Ist Teil von

• Immunology, 2015-10, Vol.146 (2), p.217-233

Ort / Verlag

England: Wiley Subscription Services, Inc

Erscheinungsjahr

2015

Quelle

Wiley Online Library - AutoHoldings Journals

Beschreibungen/Notizen

• Summary The glycophosphatidylinositol‐anchored cell surface receptor CD24 (also called heat‐stable antigen) promotes the apoptosis of progenitor and precursor B‐lymphocytes. However, the immediate proximal events that occur after engagement of CD24 in B cells are not precisely understood. Using a bioinformatics analysis of mouse (Mus musculus) gene expression data from the Immunological Genome Project, we found that known vesicle trafficking and cellular organization genes have similar expression patterns to CD24 during B‐cell development in the bone marrow. We therefore hypothesized that CD24 regulates vesicle trafficking. We first validated that antibody‐mediated engagement of CD24 induces apoptosis in the mouse WEHI‐231 cell line and mouse primary bone marrow‐derived B cells. We next found that CD24 surface protein expression is rapidly and dynamically regulated in both WEHI‐231 cells and primary immature B cells in response to engagement of CD24. The change in surface expression was not mediated by classical endocytosis or exocytosis. However, we found that CD24‐bearing plasma membrane‐derived extracellular microvesicles were released in response to CD24 engagement. Furthermore, in response to CD24 engagement we observed a clear exchange of CD24 between different populations of B cells. Hence, we show that engagement of CD24 in immature B cells results in a dynamic regulation of surface CD24 protein and a redistribution of CD24 within the population.