Details

Autor(en) / Beteiligte

• Kobayashi, Kosuke
• Suzuki, Toshiya
• Iwata, Eriko
• Nakamichi, Norihito
• Suzuki, Takamasa
• Chen, Poyu
• Ohtani, Misato
• Ishida, Takashi
• Hosoya, Hanako
• Müller, Sabine
• Leviczky, Tünde
• Pettkó-Szandtner, Aladár
• Darula, Zsuzsanna
• Iwamoto, Akitoshi
• Nomoto, Mika
• Tada, Yasuomi
• Higashiyama, Tetsuya
• Demura, Taku
• Doonan, John H
• Hauser, Marie-Theres
• Sugimoto, Keiko
• Umeda, Masaaki
• Magyar, Zoltán
• Bögre, László
• Ito, Masaki

Titel

Transcriptional repression by MYB3R proteins regulates plant organ growth

Ist Teil von

• The EMBO journal, 2015-08, Vol.34 (15), p.1992-2007

Ort / Verlag

England: Blackwell Publishing Ltd

Erscheinungsjahr

2015

Link zum Volltext

Quelle

Wiley Online Library Journals Frontfile Complete

Beschreibungen/Notizen

• In multicellular organisms, temporal and spatial regulation of cell proliferation is central for generating organs with defined sizes and morphologies. For establishing and maintaining the post‐mitotic quiescent state during cell differentiation, it is important to repress genes with mitotic functions. We found that three of the Arabidopsis MYB3R transcription factors synergistically maintain G2/M‐specific genes repressed in post‐mitotic cells and restrict the time window of mitotic gene expression in proliferating cells. The combined mutants of the three repressor‐type MYB3R genes displayed long roots, enlarged leaves, embryos, and seeds. Genome‐wide chromatin immunoprecipitation revealed that MYB3R3 binds to the promoters of G2/M‐specific genes and to E2F target genes. MYB3R3 associates with the repressor‐type E2F, E2FC, and the RETINOBLASTOMA RELATED proteins. In contrast, the activator MYB3R4 was in complex with E2FB in proliferating cells. With mass spectrometry and pairwise interaction assays, we identified some of the other conserved components of the multiprotein complexes, known as DREAM/dREAM in human and flies. In plants, these repressor complexes are important for periodic expression during cell cycle and to establish a post‐mitotic quiescent state determining organ size. Synopsis Activator and DREAM‐like repressor R1R2R3‐Myb transcription factors act coordinately to regulate mitotic target genes and organ growth in Arabidopsis thaliana. R1R2R3‐Mybs with repressor functions maintain G2/M‐specific genes repressed in post‐mitotic cells and restrict mitotic gene expression in proliferating cells. Transcriptional cell cycle regulation at G2/M impacts on plant organ growth. R1R2R3‐Myb activators and repressors form distinct multi‐protein complexes. Repressive R1R2R3‐Mybs are similar to the DREAM/dREAM complexes in human and fly. Activator and DREAM‐like repressor R1R2R3‐Myb transcription factors act coordinately to regulate mitotic target genes and organ growth in Arabidopsis thaliana.