Nanomedicine, 2015-11, Vol.11 (8), p.2013-2023
2015
Volltextzugriff (PDF)
Details
- Autor(en) / Beteiligte
- Titel
- 3D tumor tissue analogs and their orthotopic implants for understanding tumor-targeting of microenvironment-responsive nanosized chemotherapy and radiation
- Ist Teil von
- Nanomedicine, 2015-11, Vol.11 (8), p.2013-2023
- Ort / Verlag
- United States: Elsevier Inc
- Erscheinungsjahr
- 2015
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Abstract An appropriate representation of the tumor microenvironment in tumor models can have a pronounced impact on directing combinatorial treatment strategies and cancer nanotherapeutics. The present study develops a novel 3D co-culture spheroid model (3D TNBC) incorporating tumor cells, endothelial cells and fibroblasts as color-coded murine tumor tissue analogs (TTA) to better represent the tumor milieu of triple negative breast cancer in vitro . Implantation of TTA orthotopically in nude mice, resulted in enhanced growth and aggressive metastasis to ectopic sites. Subsequently, the utility of the model is demonstrated for preferential targeting of irradiated tumor endothelial cells via radiation-induced stromal enrichment of galectin-1 using anginex conjugated nanoparticles (nanobins) carrying arsenic trioxide and cisplatin. Demonstration of a multimodal nanotherapeutic system and inclusion of the biological response to radiation using an in vitro / in vivo tumor model incorporating characteristics of tumor microenvironment presents an advance in preclinical evaluation of existing and novel cancer nanotherapies. From the Clinical Editor Existing in-vivo tumor models are established by implanting tumor cells into nude mice. Here, the authors described their approach 3D spheres containing tumor cells, enodothelial cells and fibroblasts. This would mimic tumor micro-environment more realistically. This interesting 3D model should reflect more accurately tumor response to various drugs and would enable the design of new treatment modalities.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1549-9634eISSN: 1549-9642DOI: 10.1016/j.nano.2015.07.013Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4548830
- Format
- –
- Schlagworte
- 3 dimensional triple negative breast cancer (3D TNBC) model, 3D co-cultures, Animals, Antineoplastic Agents - administration & dosage, Antineoplastic Agents - therapeutic use, Arsenic Trioxide, Arsenicals - administration & dosage, Arsenicals - therapeutic use, Breast - drug effects, Breast - pathology, Breast - radiation effects, Cisplatin - administration & dosage, Cisplatin - therapeutic use, Coculture Techniques - methods, Disease Models, Animal, Drug Delivery Systems, Endothelial Cells - drug effects, Endothelial Cells - pathology, Female, Fibroblasts - drug effects, Fibroblasts - pathology, Galectin 1 - analysis, Galectin-1, Internal Medicine, Mice, Mice, Nude, Nanoparticles - chemistry, Oxides - administration & dosage, Oxides - therapeutic use, Spheroids, Cellular - drug effects, Spheroids, Cellular - pathology, Targeted nanoparticle, Triple Negative Breast Neoplasms - drug therapy, Triple Negative Breast Neoplasms - pathology, Triple Negative Breast Neoplasms - radiotherapy, Tumor cell spheroids, Tumor Cells, Cultured - drug effects, Tumor Cells, Cultured - pathology, Tumor microenvironment, Tumor Microenvironment - drug effects, Tumor Microenvironment - radiation effects, Tumor tissue analogs (TTA)