Cancer gene therapy, 2015-08, Vol.22 (8), p.387-395
2015
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- Autor(en) / Beteiligte
- Titel
- Inhibition of hedgehog signaling reduces the side population in human malignant mesothelioma cell lines
- Ist Teil von
- Cancer gene therapy, 2015-08, Vol.22 (8), p.387-395
- Ort / Verlag
- England: Nature Publishing Group
- Erscheinungsjahr
- 2015
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Deregulation of crucial embryonic pathways, including hedgehog signaling, has been frequently implicated in a variety of human cancers and is emerging as an important target for anticancer therapy. This study evaluated the potential anticancer effects of cyclopamine, a chemical inhibitor of hedgehog signaling, in human malignant mesothelioma (HMM) cell lines. Cyclopamine treatment significantly decreased the proliferation of HMM cells by promoting apoptosis and shifting the cell cycle toward dormant phase. The clonogenicity and mobility of HMM cells were significantly decreased by cyclopamine treatment. Treatment of HMM cells with cyclopamine significantly reduced the abundance of side population cells, which were measured using an assay composed of Hoechst 33342 dye staining and subsequent flow cytometry. Furthermore, the expression levels of stemness-related genes were significantly affected by cyclopamine treatment. Taken together, the present study showed that targeting hedgehog signaling could reduce a more aggressive subpopulation of the cancer cells, suggesting an alternative approach for HMM therapy.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0929-1903eISSN: 1476-5500DOI: 10.1038/cgt.2015.31Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4541142
- Format
- –
- Schlagworte
- Antineoplastic Agents, Phytogenic - pharmacology, Apoptosis - drug effects, Cell Cycle - drug effects, Cell Line, Tumor - drug effects, Cell Movement - drug effects, Cell Proliferation - drug effects, Cellular signal transduction, Development and progression, Gene Expression Regulation, Neoplastic - drug effects, Genetic aspects, Health aspects, Hedgehog proteins, Hedgehog Proteins - antagonists & inhibitors, Hedgehog Proteins - metabolism, Humans, Innovations, Lung Neoplasms - drug therapy, Lung Neoplasms - genetics, Lung Neoplasms - metabolism, Lung Neoplasms - pathology, Mesothelioma, Mesothelioma - drug therapy, Mesothelioma - genetics, Mesothelioma - metabolism, Mesothelioma - pathology, Mesothelioma, Malignant, Molecular targeted therapy, Molecular Targeted Therapy - methods, Original, Properties, Signal Transduction - drug effects, Tomatine - analogs & derivatives, Tomatine - pharmacology, Veratrum Alkaloids - pharmacology