Details

Autor(en) / Beteiligte

• Rothbart, Scott B.
• Dickson, Bradley M.
• Raab, Jesse R.
• Grzybowski, Adrian T.
• Krajewski, Krzysztof
• Guo, Angela H.
• Shanle, Erin K.
• Josefowicz, Steven Z.
• Fuchs, Stephen M.
• Allis, C. David
• Magnuson, Terry R.
• Ruthenburg, Alexander J.
• Strahl, Brian D.

Titel

An Interactive Database for the Assessment of Histone Antibody Specificity

Ist Teil von

• Molecular cell, 2015-08, Vol.59 (3), p.502-511

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2015

Quelle

MEDLINE

Beschreibungen/Notizen

• Access to high-quality antibodies is a necessity for the study of histones and their posttranslational modifications (PTMs). Here we debut the Histone Antibody Specificity Database (http://www.histoneantibodies.com), an online and expanding resource cataloging the behavior of widely used, commercially available histone antibodies by peptide microarray. This interactive web portal provides a critical resource to the biological research community that routinely uses these antibodies as detection reagents for a wide range of applications. [Display omitted] •Peptide microarrays catalog the specificity of widely used histone antibodies•Histone antibodies frequently show difficulties in detecting their intended targets•A Histone Antibody Specificity Database (http://www.histoneantibodies.com) was created•This resource facilitates more informed antibody choice for epigenetic applications Rothbart et al. use a recently developed histone peptide microarray platform to analyze the specificities of over 100 commercially available and widely used antibodies against histone posttranslational modifications. A publicly available database that is interactive and is designed to expand through user participation has been created, http://www.histoneantibodies.com.