Details

Autor(en) / Beteiligte

• Russ, Brendan E.
• Olshanksy, Moshe
• Smallwood, Heather S.
• Li, Jasmine
• Denton, Alice E.
• Prier, Julia E.
• Stock, Angus T.
• Croom, Hayley A.
• Cullen, Jolie G.
• Nguyen, Michelle L. T.
• Rowe, Stephanie
• Olson, Matthew R.
• Finkelstein, David B.
• Kelso, Anne
• Thomas, Paul G.
• Speed, Terry P.
• Rao, Sudha
• Turner, Stephen J.

Titel

Mapping histone methylation dynamics during virus-specific CD8+ T cell differentiation in response to infection

Ist Teil von

• Immunity (Cambridge, Mass.), 2014-11, Vol.41 (5), p.853-865

Erscheinungsjahr

2014

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• The molecular mechanisms that regulate the rapid transcriptional changes that occur during cytotoxic T lymphocyte (CTL) proliferation and differentiation in response to infection are poorly understood. We have utilised ChIP-seq to assess histone H3 methylation dynamics within naïve, effector and memory virus-specific T cells isolated directly ex vivo after influenza A virus infection. Our results show that within naïve T cells, co-deposition of the permissive H3K4me3 and repressive H3K27me3 modifications is a signature of gene loci associated with gene transcription, replication and cellular differentiation. Upon differentiation into effector and/or memory CTL, the majority of these gene loci lose the repressive H3K27me3 while retaining the permissive H3K4me3 modification. In contrast, immune-related effector gene promoters within naïve T cells lacked the permissive H3K4me3 modification, with acquisition of this modification occurring upon differentiation into effector/memory CTL. Thus, coordinate transcriptional regulation of CTL genes with related functions is achieved using distinct epigenetic mechanisms.