Scientific reports, 2015-06, Vol.5 (1), p.10443-10443, Article 10443
2015
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- Autor(en) / Beteiligte
- Titel
- TFH cells accumulate in mucosal tissues of humanized-DRAG mice and are highly permissive to HIV-1
- Ist Teil von
- Scientific reports, 2015-06, Vol.5 (1), p.10443-10443, Article 10443
- Ort / Verlag
- England: Nature Publishing Group
- Erscheinungsjahr
- 2015
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- CD4(+) T follicular helper cells (TFH) in germinal centers are required for maturation of B-cells. While the role of TFH-cells has been studied in blood and lymph nodes of HIV-1 infected individuals, its role in the mucosal tissues has not been investigated. We show that the gut and female reproductive tract (FRT) of humanized DRAG mice have a high level of human lymphocytes and a high frequency of TFH (CXCR5(+)PD-1(++)) and precursor-TFH (CXCR5(+)PD-1(+)) cells. The majority of TFH-cells expressed CCR5 and CXCR3 and are the most permissive to HIV-1 infection. A single low-dose intravaginal HIV-1 challenge of humanized DRAG mice results in 100% infectivity with accumulation of TFH-cells mainly in the Peyer's patches and FRT. The novel finding of TFH-cells in the FRT may contribute to the high susceptibility of DRAG mice to HIV-1 infection. This mouse model thus provides new opportunities to study TFH-cells and to evaluate HIV-1 vaccines.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 2045-2322eISSN: 2045-2322DOI: 10.1038/srep10443Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4451806
- Format
- –
- Schlagworte
- Animals, Antigens, Surface - metabolism, B-Lymphocyte Subsets - immunology, B-Lymphocyte Subsets - metabolism, CCR5 protein, CD4 antigen, CXCR3 protein, CXCR5 protein, Disease Models, Animal, Disease Susceptibility, Female, Germinal centers, Helper cells, HIV Infections - immunology, HIV Infections - virology, HIV-1 - immunology, Humans, Immunophenotyping, Inducible T-Cell Co-Stimulator Protein - metabolism, Infectivity, Interleukins - biosynthesis, Leukocyte Common Antigens - metabolism, Lymph nodes, Lymphocytes B, Lymphoid Tissue - immunology, Lymphoid Tissue - metabolism, Mice, Mice, Transgenic, Mucosa, Mucous Membrane - immunology, Mucous Membrane - metabolism, PD-1 protein, Peyer's patches, Peyer's Patches - immunology, Peyer's Patches - metabolism, Proto-Oncogene Proteins c-bcl-6 - metabolism, Receptors, CCR5 - metabolism, Receptors, CXCR3 - metabolism, Reproductive system, Rodents, T-Lymphocyte Subsets - immunology, T-Lymphocyte Subsets - metabolism, T-Lymphocyte Subsets - virology, T-Lymphocytes, Helper-Inducer - immunology, T-Lymphocytes, Helper-Inducer - metabolism, T-Lymphocytes, Helper-Inducer - virology