Angewandte Chemie International Edition, 2015-02, Vol.54 (9), p.2811-2815
International ed. in English, 2015
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Details
- Autor(en) / Beteiligte
- Titel
- Structural Characterization of O- and C-Glycosylating Variants of the Landomycin Glycosyltransferase LanGT2
- Ist Teil von
- Angewandte Chemie International Edition, 2015-02, Vol.54 (9), p.2811-2815
- Auflage
- International ed. in English
- Ort / Verlag
- Weinheim: WILEY-VCH Verlag
- Erscheinungsjahr
- 2015
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- The structures of the O‐glycosyltransferase LanGT2 and the engineered, CC bond‐forming variant LanGT2S8Ac show how the replacement of a single loop can change the functionality of the enzyme. Crystal structures of the enzymes in complex with a nonhydrolyzable nucleotide‐sugar analogue revealed that there is a conformational transition to create the binding sites for the aglycon substrate. This induced‐fit transition was explored by molecular docking experiments with various aglycon substrates. Oh to see glycosyl transfer: Crystal structures of the O‐glycosyltransferase LanGT2 and the engineered CC bond‐forming variant LanGT2S8Ac show how the replacement of a single loop can change the functionality of the enzyme. Crystal structures of the enzymes in complex with a nonhydrolyzable nucleotide‐sugar analogue revealed that there is a conformational transition to create the binding sites for the aglycon substrate.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1433-7851eISSN: 1521-3773DOI: 10.1002/anie.201409792Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4376353
- Format
- –
- Schlagworte
- Analogue, Binding sites, Bonding, C-glycosylation, carbasugars, Crystal structure, Crystallography, X-Ray, Docking, enzyme engineering, Enzymes, Friedel-Crafts alkylation, Glycosylation, glycosyltransferases, Glycosyltransferases - chemistry, Glycosyltransferases - metabolism, Molecular Docking Simulation, Protein Conformation, Protein Engineering, Structural analysis