Details

Autor(en) / Beteiligte

• Meacham, Corbin E
• Lawton, Lee N
• Soto-Feliciano, Yadira M
• Pritchard, Justin R
• Joughin, Brian A
• Ehrenberger, Tobias
• Fenouille, Nina
• Zuber, Johannes
• Williams, Richard T
• Young, Richard A
• Hemann, Michael T

Titel

A genome-scale in vivo loss-of-function screen identifies Phf6 as a lineage-specific regulator of leukemia cell growth

Ist Teil von

• Genes & development, 2015-03, Vol.29 (5), p.483-488

Ort / Verlag

United States: Cold Spring Harbor Laboratory Press

Erscheinungsjahr

2015

Quelle

MEDLINE

Beschreibungen/Notizen

• We performed a genome-scale shRNA screen for modulators of B-cell leukemia progression in vivo. Results from this work revealed dramatic distinctions between the relative effects of shRNAs on the growth of tumor cells in culture versus in their native microenvironment. Specifically, we identified many "context-specific" regulators of leukemia development. These included the gene encoding the zinc finger protein Phf6. While inactivating mutations in PHF6 are commonly observed in human myeloid and T-cell malignancies, we found that Phf6 suppression in B-cell malignancies impairs tumor progression. Thus, Phf6 is a "lineage-specific" cancer gene that plays opposing roles in developmentally distinct hematopoietic malignancies.