Details

Autor(en) / Beteiligte

• Potter, Brittney M J
• Xie, Lisa H
• Vuong, Chau
• Zhang, Jing
• Zhang, Ping
• Duan, Dehui
• Luong, Thu-Lan T
• Bandara Herath, H M T
• Dhammika Nanayakkara, N P
• Tekwani, Babu L
• Walker, Larry A
• Nolan, Christina K
• Sciotti, Richard J
• Zottig, Victor E
• Smith, Philip L
• Paris, Robert M
• Read, Lisa T
• Li, Qigui
• Pybus, Brandon S
• Sousa, Jason C
• Reichard, Gregory A
• Marcsisin, Sean R

Titel

Differential CYP 2D6 metabolism alters primaquine pharmacokinetics

Ist Teil von

• Antimicrobial agents and chemotherapy, 2015-04, Vol.59 (4), p.2380-2387

Ort / Verlag

United States: American Society for Microbiology

Erscheinungsjahr

2015

Link zum Volltext

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• Primaquine (PQ) metabolism by the cytochrome P450 (CYP) 2D family of enzymes is required for antimalarial activity in both humans (2D6) and mice (2D). Human CYP 2D6 is highly polymorphic, and decreased CYP 2D6 enzyme activity has been linked to decreased PQ antimalarial activity. Despite the importance of CYP 2D metabolism in PQ efficacy, the exact role that these enzymes play in PQ metabolism and pharmacokinetics has not been extensively studied in vivo. In this study, a series of PQ pharmacokinetic experiments were conducted in mice with differential CYP 2D metabolism characteristics, including wild-type (WT), CYP 2D knockout (KO), and humanized CYP 2D6 (KO/knock-in [KO/KI]) mice. Plasma and liver pharmacokinetic profiles from a single PQ dose (20 mg/kg of body weight) differed significantly among the strains for PQ and carboxy-PQ. Additionally, due to the suspected role of phenolic metabolites in PQ efficacy, these were probed using reference standards. Levels of phenolic metabolites were highest in mice capable of metabolizing CYP 2D6 substrates (WT and KO/KI 2D6 mice). PQ phenolic metabolites were present in different quantities in the two strains, illustrating species-specific differences in PQ metabolism between the human and mouse enzymes. Taking the data together, this report furthers understanding of PQ pharmacokinetics in the context of differential CYP 2D metabolism and has important implications for PQ administration in humans with different levels of CYP 2D6 enzyme activity.