Details

Autor(en) / Beteiligte

• Kinjyo, Ichiko
• Qin, Jim
• Tan, Sioh-Yang
• Wellard, Cameron J
• Mrass, Paulus
• Ritchie, William
• Doi, Atsushi
• Cavanagh, Lois L
• Tomura, Michio
• Sakaue-Sawano, Asako
• Kanagawa, Osami
• Miyawaki, Atsushi
• Hodgkin, Philip D
• Weninger, Wolfgang

Titel

Real-time tracking of cell cycle progression during CD8+ effector and memory T-cell differentiation

Ist Teil von

• Nature communications, 2015-02, Vol.6 (1), p.6301-6301, Article 6301

Ort / Verlag

England: Nature Publishing Group

Erscheinungsjahr

2015

Quelle

MEDLINE

Beschreibungen/Notizen

• The precise pathways of memory T-cell differentiation are incompletely understood. Here we exploit transgenic mice expressing fluorescent cell cycle indicators to longitudinally track the division dynamics of individual CD8(+) T cells. During influenza virus infection in vivo, naive T cells enter a CD62L(intermediate) state of fast proliferation, which continues for at least nine generations. At the peak of the anti-viral immune response, a subpopulation of these cells markedly reduces their cycling speed and acquires a CD62L(hi) central memory cell phenotype. Construction of T-cell family division trees in vitro reveals two patterns of proliferation dynamics. While cells initially divide rapidly with moderate stochastic variations of cycling times after each generation, a slow-cycling subpopulation displaying a CD62L(hi) memory phenotype appears after eight divisions. Phenotype and cell cycle duration are inherited by the progeny of slow cyclers. We propose that memory precursors cell-intrinsically modulate their proliferative activity to diversify differentiation pathways.