Details

Autor(en) / Beteiligte

• Bass, V.
• Gordon, C.J.
• Jarema, K.A.
• MacPhail, R.C.
• Cascio, W.E.
• Phillips, P.M.
• Ledbetter, A.D.
• Schladweiler, M.C.
• Andrews, D.
• Miller, D.
• Doerfler, D.L.
• Kodavanti, U.P.

Titel

Ozone induces glucose intolerance and systemic metabolic effects in young and aged brown Norway rats

Ist Teil von

• Toxicology and applied pharmacology, 2013-12, Vol.273 (3), p.551-560

Ort / Verlag

Amsterdam: Elsevier Inc

Erscheinungsjahr

2013

Quelle

MEDLINE

Beschreibungen/Notizen

• Air pollutants have been associated with increased diabetes in humans. We hypothesized that ozone would impair glucose homeostasis by altering insulin signaling and/or endoplasmic reticular (ER) stress in young and aged rats. One, 4, 12, and 24month old Brown Norway (BN) rats were exposed to air or ozone, 0.25 or 1.0ppm, 6h/day for 2days (acute) or 2d/week for 13weeks (subchronic). Additionally, 4month old rats were exposed to air or 1.0ppm ozone, 6h/day for 1 or 2days (time-course). Glucose tolerance tests (GTT) were performed immediately after exposure. Serum and tissue biomarkers were analyzed 18h after final ozone for acute and subchronic studies, and immediately after each day of exposure in the time-course study. Age-related glucose intolerance and increases in metabolic biomarkers were apparent at baseline. Acute ozone caused hyperglycemia and glucose intolerance in rats of all ages. Ozone-induced glucose intolerance was reduced in rats exposed for 13weeks. Acute, but not subchronic ozone increased α2-macroglobulin, adiponectin and osteopontin. Time-course analysis indicated glucose intolerance at days 1 and 2 (2>1), and a recovery 18h post ozone. Leptin increased day 1 and epinephrine at all times after ozone. Ozone tended to decrease phosphorylated insulin receptor substrate-1 in liver and adipose tissues. ER stress appeared to be the consequence of ozone induced acute metabolic impairment since transcriptional markers of ER stress increased only after 2days of ozone. In conclusion, acute ozone exposure induces marked systemic metabolic impairments in BN rats of all ages, likely through sympathetic stimulation. •Air pollutants have been associated with increased diabetes in humans.•Acute ozone exposure produces profound metabolic alterations in rats.•Age influences metabolic risk factors in aging BN rats.•Acute metabolic effects are reversible and repeated exposure reduces these effects.•Ozone metabolic effects are only slightly exacerbated in geriatric rats.