Scientific reports, 2015-02, Vol.5 (1), p.8518-8518, Article 8518
2015
Details
- Autor(en) / Beteiligte
- Titel
- Impact of Sox9 dosage and Hes1-mediated Notch signaling in controlling the plasticity of adult pancreatic duct cells in mice
- Ist Teil von
- Scientific reports, 2015-02, Vol.5 (1), p.8518-8518, Article 8518
- Ort / Verlag
- England: Nature Publishing Group
- Erscheinungsjahr
- 2015
- Link zum Volltext
- Quelle
- Free E-Journal (出版社公開部分のみ）
- Beschreibungen/Notizen
- In the adult pancreas, there has been a long-standing dispute as to whether stem/precursor populations that retain plasticity to differentiate into endocrine or acinar cell types exist in ducts. We previously reported that adult Sox9-expressing duct cells are sufficiently plastic to supply new acinar cells in Sox9-IRES-CreERT2 knock-in mice. In the present study, using Sox9-IRES-CreERT2 knock-in mice as a model, we aimed to analyze how plasticity is controlled in adult ducts. Adult duct cells in these mice express less Sox9 than do wild-type mice but Hes1 equally. Acinar cell differentiation was accelerated by Hes1 inactivation, but suppressed by NICD induction in adult Sox9-expressing cells. Quantitative analyses showed that Sox9 expression increased with the induction of NICD but did not change with Hes1 inactivation, suggesting that Notch regulates Hes1 and Sox9 in parallel. Taken together, these findings suggest that Hes1-mediated Notch activity determines the plasticity of adult pancreatic duct cells and that there may exist a dosage requirement of Sox9 for keeping the duct cell identity in the adult pancreas. In contrast to the extended capability of acinar cell differentiation by Hes1 inactivation, we obtained no evidence of islet neogenesis from Hes1-depleted duct cells in physiological or PDL-induced injured conditions.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 2045-2322eISSN: 2045-2322DOI: 10.1038/srep08518Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4330537
- Format
- –
- Schlagworte
- Acinar cells, Age Factors, Animals, Basic Helix-Loop-Helix Transcription Factors - metabolism, Cell Differentiation - genetics, Cell Plasticity, Gene Dosage, Gene Expression, Homeodomain Proteins - metabolism, Mice, Mice, Transgenic, Models, Biological, Notch protein, Pancreas, Pancreatic Ducts - cytology, Pancreatic Ducts - metabolism, Plasticity, Receptors, Notch - metabolism, Rodents, Signal Transduction, Sox9 protein, SOX9 Transcription Factor - genetics, SOX9 Transcription Factor - metabolism, Transcription Factor HES-1, Transcription Factors - genetics, Transcription Factors - metabolism