Details

Autor(en) / Beteiligte

• Cleghorn, Whitney M
• Branch, Kevin M
• Kook, Seunghyi
• Arnette, Christopher
• Bulus, Nada
• Zent, Roy
• Kaverina, Irina
• Gurevich, Eugenia V
• Weaver, Alissa M
• Gurevich, Vsevolod V
• Parent, Carole

Titel

Arrestins regulate cell spreading and motility via focal adhesion dynamics

Ist Teil von

• Molecular biology of the cell, 2015-02, Vol.26 (4), p.622-635

Ort / Verlag

United States: The American Society for Cell Biology

Erscheinungsjahr

2015

Quelle

MEDLINE

Beschreibungen/Notizen

• Focal adhesions (FAs) play a key role in cell attachment, and their timely disassembly is required for cell motility. Both microtubule-dependent targeting and recruitment of clathrin are critical for FA disassembly. Here we identify nonvisual arrestins as molecular links between microtubules and clathrin. Cells lacking both nonvisual arrestins showed excessive spreading on fibronectin and poly-d-lysine, increased adhesion, and reduced motility. The absence of arrestins greatly increases the size and lifespan of FAs, indicating that arrestins are necessary for rapid FA turnover. In nocodazole washout assays, FAs in arrestin-deficient cells were unresponsive to disassociation or regrowth of microtubules, suggesting that arrestins are necessary for microtubule targeting-dependent FA disassembly. Clathrin exhibited decreased dynamics near FA in arrestin-deficient cells. In contrast to wild-type arrestins, mutants deficient in clathrin binding did not rescue the phenotype. Collectively the data indicate that arrestins are key regulators of FA disassembly linking microtubules and clathrin.