Details

Autor(en) / Beteiligte

• Daigle, Francois
• Lerat, Sylvain
• Bucca, Giselda
• Sanssouci, Édith
• Smith, Colin P
• Malouin, Francois
• Beaulieu, Carole
• Schneewind, O.

Titel

terD Domain-Encoding Gene (SCO2368) Is Involved in Calcium Homeostasis and Participates in Calcium Regulation of a DosR-Like Regulon in Streptomyces coelicolor

Ist Teil von

• Journal of bacteriology, 2015-03, Vol.197 (5), p.913-923

Ort / Verlag

United States: American Society for Microbiology

Erscheinungsjahr

2015

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• Although Streptomyces coelicolor is not resistant to tellurite, it possesses several TerD domain-encoding (tdd) genes of unknown function. To elucidate the function of tdd8 , the transcriptomes of S. coelicolor strain M145 and of a tdd8 deletion mutant derivative (the Δ tdd8 strain) were compared. Several orthologs of Mycobacterium tuberculosis genes involved in dormancy survival were upregulated in the deletion mutant at the visual onset of prodiginine production. These genes are organized in a putative redox stress response cluster comprising two large loci. A binding motif similar to the dormancy survival regulator (DosR) binding site of M. tuberculosis has been identified in the upstream sequences of most genes in these loci. A predicted role for these genes in the redox stress response is supported by the low NAD ⁺/NADH ratio in the Δ tdd8 strain. This S. coelicolor gene cluster was shown to be induced by hypoxia and NO stress. While the tdd8 deletion mutant (the Δ tdd8 strain) was unable to maintain calcium homeostasis in a calcium-depleted medium, the addition of Ca ²⁺ in Δ tdd8 culture medium reduced the expression of several genes of the redox stress response cluster. The results shown in this work are consistent with Tdd8 playing a significant role in calcium homeostasis and redox stress adaptation.