Details

Autor(en) / Beteiligte

• Adriaenssens, Evelien M
• Lehman, Susan M
• Vandersteegen, Katrien
• Vandenheuvel, Dieter
• Philippe, Didier L
• Cornelissen, Anneleen
• Clokie, Martha R.J
• García, Andrés J
• De Proft, Maurice
• Maes, Martine
• Lavigne, Rob

Titel

CIM® monolithic anion-exchange chromatography as a useful alternative to CsCl gradient purification of bacteriophage particles

Ist Teil von

• Virology (New York, N.Y.), 2012-12, Vol.434 (2), p.265-270

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2012

Quelle

ScienceDirect

Beschreibungen/Notizen

• Abstract The use of anion-exchange chromatography was investigated as an alternative method to concentrate and purify bacterial viruses, and parameters for different bacteriophages were compared. Chromatography was performed with Convective Interactive Media® monoliths, with three different volumes and two matrix chemistries. Eleven morphologically distinct phages were tested, infecting five different bacterial species. For each of the phages tested, a protocol was optimized, including the choice of column chemistry, loading, buffer and elution conditions. The capacity and recovery of the phages on the columns varied considerably between phages. We conclude that anion-exchange chromatography with monoliths is a valid alternative to the more traditional CsCl purification, has upscaling advantages, but it requires more extensive optimization.