Toxicological sciences, 2015-01, Vol.143 (1), p.136-146
2015
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Details
- Autor(en) / Beteiligte
- Titel
- Formation of a protein corona on silver nanoparticles mediates cellular toxicity via scavenger receptors
- Ist Teil von
- Toxicological sciences, 2015-01, Vol.143 (1), p.136-146
- Ort / Verlag
- United States: Oxford University Press
- Erscheinungsjahr
- 2015
- Quelle
- Oxford Journals 2020 Medicine
- Beschreibungen/Notizen
- Addition of a protein corona (PC) or protein adsorption layer on the surface of nanomaterials following their introduction into physiological environments may modify their activity, bio-distribution, cellular uptake, clearance, and toxicity. We hypothesize that silver nanoparticles (AgNPs) will associate with proteins common to human serum and cell culture media forming a PC that will impact cell activation and cytotoxicity. Furthermore, the role of scavenger receptor BI (SR-BI) in mediating this toxicity was evaluated. Citrate-suspended 20 nm AgNPs were incubated with human serum albumin (HSA), bovine serum albumin (BSA), high-density lipoprotein (HDL), or water (control) to form a PC. AgNPs associated with each protein (HSA, BSA, and HDL) forming PCs as assessed by electron microscopy, hyperspectral analysis, ζ-potential, and hydrodynamic size. Addition of the PC decreased uptake of AgNPs by rat lung epithelial and rat aortic endothelial cells. Hyperspectral analysis demonstrated a loss of the AgNP PC following internalization. Cells demonstrated concentration-dependent cytotoxicity following exposure to AgNPs with or without PCs (0, 6.25, 12.5, 25 or 50 μg/ml). All PC-coated AgNPs were found to activate cells by inducing IL-6 mRNA expression. A small molecule SR-BI inhibitor was utilized to determine the role of SR-BI in the observed effects. Pretreatment with the SR-BI inhibitor decreased internalization of AgNPs with or without PCs, and reduced both cytotoxicity and IL-6 mRNA expression. This study characterizes the formation of a PC on AgNPs and demonstrates its influence on cytotoxicity and cell activation through a cell surface receptor.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1096-6080eISSN: 1096-0929DOI: 10.1093/toxsci/kfu217Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4274384
- Format
- –
- Schlagworte
- Adsorption, Animals, Aorta - drug effects, Aorta - metabolism, Aorta - pathology, Cell Survival - drug effects, Cells, Cultured, Dose-Response Relationship, Drug, Endothelial Cells - drug effects, Endothelial Cells - metabolism, Endothelial Cells - pathology, Epithelial Cells - drug effects, Epithelial Cells - metabolism, Epithelial Cells - pathology, Interleukin-6 - genetics, Interleukin-6 - metabolism, Lipoproteins, HDL - chemistry, Lipoproteins, HDL - metabolism, Lung - drug effects, Lung - metabolism, Lung - pathology, Metal Nanoparticles - chemistry, Metal Nanoparticles - toxicity, Particle Size, Protein Conformation, Protein Corona - chemistry, Protein Corona - metabolism, Rats, RNA, Messenger - metabolism, Scavenger Receptors, Class B - antagonists & inhibitors, Scavenger Receptors, Class B - metabolism, Serum Albumin - chemistry, Serum Albumin - metabolism, Serum Albumin, Bovine - chemistry, Serum Albumin, Bovine - metabolism, Serum Albumin, Human, Silver - chemistry, Silver - toxicity, Silver Nanoparticle Protein Coating Toxicity, Surface Properties, Up-Regulation