Details

Autor(en) / Beteiligte

• Itoe, Maurice A.
• Sampaio, Júlio L.
• Cabal, Ghislain G.
• Real, Eliana
• Zuzarte-Luis, Vanessa
• March, Sandra
• Bhatia, Sangeeta N.
• Frischknecht, Friedrich
• Thiele, Christoph
• Shevchenko, Andrej
• Mota, Maria M.

Titel

Host Cell Phosphatidylcholine Is a Key Mediator of Malaria Parasite Survival during Liver Stage Infection

Ist Teil von

• Cell host & microbe, 2014-12, Vol.16 (6), p.778-786

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2014

Quelle

Elektronische Zeitschriftenbibliothek

Beschreibungen/Notizen

• During invasion, Plasmodium, the causative agent of malaria, wraps itself in a parasitophorous vacuole membrane (PVM), which constitutes a critical interface between the parasite and its host cell. Within hepatocytes, each Plasmodium sporozoite generates thousands of new parasites, creating high demand for lipids to support this replication and enlarge the PVM. Here, a global analysis of the total lipid repertoire of Plasmodium-infected hepatocytes reveals an enrichment of neutral lipids and the major membrane phospholipid, phosphatidylcholine (PC). While infection is unaffected in mice deficient in key enzymes involved in neutral lipid synthesis and lipolysis, ablation of rate-limiting enzymes in hepatic PC biosynthetic pathways significantly decreases parasite numbers. Host PC is taken up by both P. berghei and P. falciparum and is necessary for correct localization of parasite proteins to the PVM, which is essential for parasite survival. Thus, Plasmodium relies on the abundance of these lipids within hepatocytes to support infection. [Display omitted] •Lipid composition of P. berghei-infected hepatocytes is altered during infection•Plasmodium liver stage infection does not require de-novo-synthesized neutral lipids•Plasmodium takes up host phosphatidylcholine (PC), which associates with the PVM•Host cell de novo PC synthesis contributes to PVM integrity and parasite survival Plasmodium replication in hepatocytes requires abundant lipid resources. By analyzing the lipidome of P. berghei-infected cells, Itoe et al. reveal enrichment of phosphatidylcholine, a major membrane phospholipid. Targeted silencing of host genes involved in de novo phosphatidylcholine synthesis show that these pathways are critical for Plasmodium liver-stage infection.